BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18446

Title: Sequence specific 1H, 13C and 15N resonance assignments of an intrinsically unstructured -Crystallin from Hahella chejuensis   PubMed: 22836948

Deposition date: 2012-05-07 Original release date: 2012-09-14

Authors: Ramanujam, Venkatraman; Patel, Sunita; Srivastava, Atul; Sharma, Yogendra; Chary, Kandala

Citation: Ramanujam, Venkatraman; Patel, Sunita; Srivastava, Atul; Sharma, Yogendra; Chary, Kandala. "Backbone 1H, 13C and 15N resonance assignments of an intrinsically unstructured -crystallin from Hahella chejuensis."  Biomol. NMR Assignments 7, 221-224 (2013).

Assembly members:
Apoform_Hahellin, polymer, 92 residues, 10249.3 Da.

Natural source:   Common Name: Hahella chejuensis   Taxonomy ID: 158327   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Hahella chejuensis

Experimental source:   Production method: recombinant technology   Host organism: Hahella chejuensis

Entity Sequences (FASTA):
Apoform_Hahellin: MGEKTVKLYEDTHFKGYSVE LPVGDYNLSSLISRGALNDD LSSARVPSGLRLEVFQHNNF KGVRDFYTSDAAELSRDNDA SSVRVSKMETTN

Data sets:
Data typeCount
13C chemical shifts222
15N chemical shifts71
1H chemical shifts71

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Apoform of Hahellin1

Entities:

Entity 1, Apoform of Hahellin 92 residues - 10249.3 Da.

1   METGLYGLULYSTHRVALLYSLEUTYRGLU
2   ASPTHRHISPHELYSGLYTYRSERVALGLU
3   LEUPROVALGLYASPTYRASNLEUSERSER
4   LEUILESERARGGLYALALEUASNASPASP
5   LEUSERSERALAARGVALPROSERGLYLEU
6   ARGLEUGLUVALPHEGLNHISASNASNPHE
7   LYSGLYVALARGASPPHETYRTHRSERASP
8   ALAALAGLULEUSERARGASPASNASPALA
9   SERSERVALARGVALSERLYSMETGLUTHR
10   THRASN

Samples:

sample_1: Apoform Hahellin, [U-100% 13C; U-100% 15N], 0.7 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.05 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - processing

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

BMRB 15743 16777
PDB
GB ABC29838
REF WP_011396907

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts