BMRB Entry 18486
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18486
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for reduced form of sulfiredoxin extract from Saccharomyces cerevisiae PubMed: 21237158
Deposition date: 2012-05-29 Original release date: 2012-07-02
Authors: Averlant-Petit, Marie-Christine; Beaufils, Chrystel; Rahuel-Clermont, Sophie; Branlant, Guy; Kriznik, Alexandre; Modh-Yahaya, Normi; Roussel, Xavier; Legrand, Baptiste
Citation: Roussel, Xavier; Boukhenouna, Samia; Rahuel-Clermont, Sophie; Branlant, Guy. "The rate-limiting step of sulfiredoxin is associated with the transfer of the gamma-phosphate of ATP to the sulfinic acid of overoxidized typical 2-Cys peroxiredoxins" FEBS Lett. 585, 574-578 (2011).
Assembly members:
Reduced_Sulfiredoxin, polymer, 127 residues, Formula weight is not available
Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Reduced_Sulfiredoxin: MSLQSNSVKPTEIPLSEIRR
PLAPVLDPQKIDAMVATMKG
IPTASKTCSLEQAEAAASAG
ELPPVDVLGVRVKGQTLYYA
FGGCHRLQAYDRRARETQNA
AFPVRCRVLPATPRQIRMYL
GSSLDIE
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 356 |
15N chemical shifts | 108 |
1H chemical shifts | 605 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Reduced Sulfiredoxin | 1 |
Entities:
Entity 1, Reduced Sulfiredoxin 127 residues - Formula weight is not available
1 | MET | SER | LEU | GLN | SER | ASN | SER | VAL | LYS | PRO | ||||
2 | THR | GLU | ILE | PRO | LEU | SER | GLU | ILE | ARG | ARG | ||||
3 | PRO | LEU | ALA | PRO | VAL | LEU | ASP | PRO | GLN | LYS | ||||
4 | ILE | ASP | ALA | MET | VAL | ALA | THR | MET | LYS | GLY | ||||
5 | ILE | PRO | THR | ALA | SER | LYS | THR | CYS | SER | LEU | ||||
6 | GLU | GLN | ALA | GLU | ALA | ALA | ALA | SER | ALA | GLY | ||||
7 | GLU | LEU | PRO | PRO | VAL | ASP | VAL | LEU | GLY | VAL | ||||
8 | ARG | VAL | LYS | GLY | GLN | THR | LEU | TYR | TYR | ALA | ||||
9 | PHE | GLY | GLY | CYS | HIS | ARG | LEU | GLN | ALA | TYR | ||||
10 | ASP | ARG | ARG | ALA | ARG | GLU | THR | GLN | ASN | ALA | ||||
11 | ALA | PHE | PRO | VAL | ARG | CYS | ARG | VAL | LEU | PRO | ||||
12 | ALA | THR | PRO | ARG | GLN | ILE | ARG | MET | TYR | LEU | ||||
13 | GLY | SER | SER | LEU | ASP | ILE | GLU |
Samples:
sample_1: Reduced Sulfiredoxin, [U-15N], 0.55 mM; H2O 95%; D2O 5%; NaPi 50 mM; NaCl 100 mM
sample_2: Reduced Sulfiredoxin, [U-13C; U-15N], 0.55 mM; H2O 95%; D2O 5%; NaPi 50 mM; NaCl 100 mM
sample_3: Reduced Sulfiredoxin, [U-13C; U-15N; U-2H], 0.55 mM; H2O 95%; D2O 5%; NaPi 50 mM; NaCl 100 mM
sample_conditions_1: ionic strength: 150 mM; pH: 6; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HNHA | sample_1 | isotropic | sample_conditions_1 |
3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
Software:
ARIA, Linge, O, . - chemical shift assignment
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker Avance 900 MHz
- Bruker DRX 800 MHz
- Bruker Avance 950 MHz
Related Database Links:
BMRB | 18488 |
DBJ | GAA24640 |
EMBL | CAA81924 CAY81004 |
GB | AAS56676 AHY76158 AJP39952 AJS30150 AJS30452 |
REF | NP_012837 |
SP | P36077 |
TPG | DAA09072 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts