BMRB Entry 18528

Name: 1H chemical shifts for the C-terminal alfa-tubulin 404-451 fragment
Published: 2012-07-13

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18528

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: 1H chemical shifts for the C-terminal alfa-tubulin 404-451 fragment PubMed: 10211825

Deposition date: 2012-06-17 Original release date: 2012-07-13

Authors: Jimenez, M. Angeles; Andreu, Jose

Citation: Andreu, M. Angeles; Evangelio, Juan; Aranda, Carlos; Lopez-Brauet, Adamari; Andreu, David; Rico, Manuel; Lagos, Rosalba; Andreu, Jose; Monasterio, Octavio. "Helicity of alpha(404-451) and bet(394-445) tubulin C-terminal recombinant peptides" Protein Sci. 8, 788-799 (1999).

Assembly members:
alpha_tubulin_404-451, polymer, 48 residues, Formula weight is not available

Natural source: Common Name: Humans Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
alpha_tubulin_404-451: FVHWYVGEGMEEGEFSEARE DMAALEKDYEEVGVDSVEGE GEEEGEEY

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	290

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	alpha tubulin 404-451	1

Entities:

Entity 1, alpha tubulin 404-451 48 residues - Formula weight is not available

1

PHE

VAL

HIS

TRP

TYR

VAL

GLY

GLU

GLY

MET

2

GLU

GLY

GLU

PHE

SER

GLU

ALA

ARG

GLU

3

ASP

MET

ALA

LEU

GLU

LYS

ASP

TYR

GLU

4

GLU

VAL

GLY

VAL

ASP

SER

VAL

GLU

GLY

GLU

5

GLY

GLU

GLY

GLU

TYR

Samples:

sample_1: alpha tubulin 404-451 2 mM; H2O 63%; D2O, [U-100% 2H], 7%; TFE, [U-99% 2H], 30 v/v; TSP 0.2 mM

sample_conditions_1: ionic strength: 0 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

xwinnmr, Bruker Biospin - processing

NMR spectrometers:

Bruker AMX 600 MHz