BMRB Entry 18578
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR18578
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Title: Chemical shift assignments of the connexin45 carboxyl terminal domain: monomer and dimer conformations. PubMed: 23070843
Deposition date: 2012-07-06 Original release date: 2012-10-18
Authors: Kopanic, Jennifer; Sorgen, Paul
Citation: Kopanic, Jennifer; Sorgen, Paul. "Chemical shift assignments of the connexin45 carboxyl terminal domain: monomer and dimer conformations." Biomol. NMR Assignments 7, 293-297 (2013).
Assembly members:
Connexin45_Carboxyl_Terminal_Domain, polymer, 134 residues, 15005.5 Da.
Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Connexin45_Carboxyl_Terminal_Domain: GSKRRELDDPGAYNYPFTWN
TPSAPPGYNIAVKPDQIQYT
ELSNAKIAYKQNKANIAQEQ
QYGSHEEHLPADLETLQREI
RMAQERLDLAIQAYHHQNNP
HGPREKKAKVGSKSGSNKSS
ISSKSGDGKTSVWI
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 505 |
15N chemical shifts | 123 |
1H chemical shifts | 707 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Connexin45 Carboxyl Terminal Domain | 1 |
Entities:
Entity 1, Connexin45 Carboxyl Terminal Domain 134 residues - 15005.5 Da.
1 | GLY | SER | LYS | ARG | ARG | GLU | LEU | ASP | ASP | PRO | ||||
2 | GLY | ALA | TYR | ASN | TYR | PRO | PHE | THR | TRP | ASN | ||||
3 | THR | PRO | SER | ALA | PRO | PRO | GLY | TYR | ASN | ILE | ||||
4 | ALA | VAL | LYS | PRO | ASP | GLN | ILE | GLN | TYR | THR | ||||
5 | GLU | LEU | SER | ASN | ALA | LYS | ILE | ALA | TYR | LYS | ||||
6 | GLN | ASN | LYS | ALA | ASN | ILE | ALA | GLN | GLU | GLN | ||||
7 | GLN | TYR | GLY | SER | HIS | GLU | GLU | HIS | LEU | PRO | ||||
8 | ALA | ASP | LEU | GLU | THR | LEU | GLN | ARG | GLU | ILE | ||||
9 | ARG | MET | ALA | GLN | GLU | ARG | LEU | ASP | LEU | ALA | ||||
10 | ILE | GLN | ALA | TYR | HIS | HIS | GLN | ASN | ASN | PRO | ||||
11 | HIS | GLY | PRO | ARG | GLU | LYS | LYS | ALA | LYS | VAL | ||||
12 | GLY | SER | LYS | SER | GLY | SER | ASN | LYS | SER | SER | ||||
13 | ILE | SER | SER | LYS | SER | GLY | ASP | GLY | LYS | THR | ||||
14 | SER | VAL | TRP | ILE |
Samples:
monomer_sample: Connexin45 Carboxyl Terminal Domain, [U-98% 13C; U-98% 15N], 0.9-1.2 mM; potassium chloride 2.7 mM; phosphate 11.9 mM; sodium chloride 137 mM; acetonitrile, [U-100% 2H], 30%; H2O 90%; D2O 10%
monomer_sample_conditions: ionic strength: 151.6 mM; pH: 5.8; pressure: 1 atm; temperature: 310 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | monomer_sample | isotropic | monomer_sample_conditions |
2D 1H-13C HSQC | monomer_sample | isotropic | monomer_sample_conditions |
3D HNCACB | monomer_sample | isotropic | monomer_sample_conditions |
3D CBCA(CO)NH | monomer_sample | isotropic | monomer_sample_conditions |
3D HNCO | monomer_sample | isotropic | monomer_sample_conditions |
3D HN(CO)CA | monomer_sample | isotropic | monomer_sample_conditions |
3D HCCH-TOCSY | monomer_sample | isotropic | monomer_sample_conditions |
3D 1H-15N TOCSY | monomer_sample | isotropic | monomer_sample_conditions |
3D 1H-15N NOESY | monomer_sample | isotropic | monomer_sample_conditions |
3D 1H-13C NOESY | monomer_sample | isotropic | monomer_sample_conditions |
3D C(CO)NH | monomer_sample | isotropic | monomer_sample_conditions |
3D H(CCO)NH | monomer_sample | isotropic | monomer_sample_conditions |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
BMRB | 18577 |
DBJ | BAC34953 |
EMBL | CAA44814 CAC21557 |
GB | AAH50840 AAH71230 AAI66405 AAN17802 AAR98617 |
REF | NP_001078850 NP_001152854 NP_001152855 NP_001233715 NP_001268477 |
SP | A4GG66 P28229 Q6PYT3 Q6R4A8 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts