BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18645

Title: NMR solution structure of PawS Derived Peptide 7 (PDP-7)   PubMed: 24681618

Deposition date: 2012-08-06 Original release date: 2013-08-15

Authors: Elliott, Alysha; Mylne, Joshua; Rosengren, Johan

Citation: Elliott, Alysha; Delay, Christina; Liu, Huanle; Phua, Zaiyang; Rosengren, K. Johan; Benfield, Aurelie; Panero, Jose; Colgrave, Michelle; Jayasena, Achala; Dunse, Kerry; Anderson, Marilyn; Schilling, Edward; Ortiz-Barrientos, Daniel; Craik, David; Mylne, Joshua. "Evolutionary Origins of a Bioactive Peptide Buried within Preproalbumin."  Plant Cell 26, 981-995 (2014).

Assembly members:
PDP-7, polymer, 15 residues, 1571.825 Da.

Natural source:   Common Name: Otopappus epaleaceus   Taxonomy ID: 243770   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Otopappus epaleaceus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
PDP-7: GHCIPTTSGPICLRD

Data sets:
Data typeCount
13C chemical shifts41
1H chemical shifts94

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PawS Derived Peptide 7 (PDP-7)1

Entities:

Entity 1, PawS Derived Peptide 7 (PDP-7) 15 residues - 1571.825 Da.

1   GLYHISCYSILEPROTHRTHRSERGLYPRO
2   ILECYSLEUARGASP

Samples:

sample_1: PDP-7 3 mg/mL; DMSO 10%; H2O 80%; D2O 10%

sample_2: PDP-7 3 mg/mL; DMSO 10%; D2O 90%

sample_conditions_1: ionic strength: 0 M; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
2D 1H-1H ECOSYsample_2isotropicsample_conditions_1

Software:

TOPSPIN v3.0, Bruker Biospin - collection, processing

XEASY, Bartels et al. - data analysis

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 500 MHz

Related Database Links:

GB JX262730 AFQ93661
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