BMRB Entry 18689
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18689
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Title: Backbone 1H, 13C, and 15N backbone resonance assignments of the TPR2A domain of mouse STI1 PubMed: 23070844
Deposition date: 2012-08-30 Original release date: 2014-02-19
Authors: Maciejewski, Andrzej; Choy, Wing-Yiu
Citation: Maciejewski, Andrzej; Prado, Marco; Choy, Wing-Yiu. "(1)H, (15)N and (13)C backbone resonance assignments of the TPR1 and TPR2A domains of mouse STI1." Biomol. NMR Assignments 7, 305-310 (2013).
Assembly members:
tpr2a, polymer, 137 residues, Formula weight is not available
Natural source: Common Name: House mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 252 |
| 15N chemical shifts | 132 |
| 1H chemical shifts | 132 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | tpr2a | 1 |
Entities:
Entity 1, tpr2a 137 residues - Formula weight is not available
Residue 216 is a non-native glycine result of cleavage of the affinity tag by TEV protease
| 1 | GLY | ASP | LEU | PRO | GLU | ASN | LYS | LYS | GLN | ALA | ||||
| 2 | LEU | LYS | GLU | LYS | GLU | LEU | GLY | ASN | ASP | ALA | ||||
| 3 | TYR | LYS | LYS | LYS | ASP | PHE | ASP | LYS | ALA | LEU | ||||
| 4 | LYS | HIS | TYR | ASP | ARG | ALA | LYS | GLU | LEU | ASP | ||||
| 5 | PRO | THR | ASN | MET | THR | TYR | ILE | THR | ASN | GLN | ||||
| 6 | ALA | ALA | VAL | HIS | PHE | GLU | LYS | GLY | ASP | TYR | ||||
| 7 | ASN | LYS | CYS | ARG | GLU | LEU | CYS | GLU | LYS | ALA | ||||
| 8 | ILE | GLU | VAL | GLY | ARG | GLU | ASN | ARG | GLU | ASP | ||||
| 9 | TYR | ARG | GLN | ILE | ALA | LYS | ALA | TYR | ALA | ARG | ||||
| 10 | ILE | GLY | ASN | SER | TYR | PHE | LYS | GLU | GLU | LYS | ||||
| 11 | TYR | LYS | ASP | ALA | ILE | HIS | PHE | TYR | ASN | LYS | ||||
| 12 | SER | LEU | ALA | GLU | HIS | ARG | THR | PRO | ASP | VAL | ||||
| 13 | LEU | LYS | LYS | CYS | GLN | GLN | ALA | GLU | LYS | ILE | ||||
| 14 | LEU | LYS | GLU | GLN | GLU | ARG | LEU |
Samples:
sample_1: sodium phosphate 50 mM; sodium chloride 50 mM; DTT 1 mM; DSS 100 uM; tpr2a, [U-13C; U-15N], 0.5 uM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 150 mM; pH: 7; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA, Keller and Wuthrich - chemical shift assignment
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts