BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18784

Title: SOLUTION NMR STRUCTURE OF ASTEROPSIN C FROM A MARINE SPONGE ASTEROPUS SP.   PubMed: 23225198

Deposition date: 2012-10-12 Original release date: 2013-10-21

Authors: Li, Huayue; Bowling, John; Hamann, Mark; Jung, Jee

Citation: Temel, Deniz; Dutta, Kaushik; Ghose, Ranajeet. "Sequence-specific backbone (1)H, (13)C and (15)N assignments of the catalytic domain of the Escherichia coli protein tyrosine kinase, Wzc."  Biomol. NMR Assignments ., .-. (2012).

Assembly members:
ABU8-3, polymer, 35 residues, 3773.257 Da.

Natural source:   Common Name: Sponges   Taxonomy ID: 350938   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Asteropus sp.

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
ABU8-3: XDCPGEGEQCDVEFNPCCPP LTCIPGDPYGICYII

Data sets:
Data typeCount
13C chemical shifts114
1H chemical shifts222

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ABU8-31

Entities:

Entity 1, ABU8-3 35 residues - 3773.257 Da.

1   PCAASPCYSPROGLYGLUGLYGLUGLNCYS
2   ASPVALGLUPHEASNPROCYSCYSPROPRO
3   LEUTHRCYSILEPROGLYASPPROTYRGLY
4   ILECYSTYRILEILE

Samples:

sample_1: ABU8-3 10 mM; CD3OH 100%

ABU8-3_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicABU8-3_conditions_1
2D 1H-1H TOCSYsample_1isotropicABU8-3_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicABU8-3_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicABU8-3_conditions_1
2D 1H-1H NOESYsample_1isotropicABU8-3_conditions_1

Software:

CNS v1.3, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

  • Varian INOVA 900 MHz
  • Varian INOVA 500 MHz

Related Database Links:

BMRB 18965
PDB