BMRB Entry 18820
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18820
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Title: 1H, 13C and 15N assignments of the Apo-acyl carrier protein-1 of Pseudomonas aeruginosa. PubMed: 21152998
Deposition date: 2012-11-05 Original release date: 2012-12-04
Authors: Parnham, Stuart; Duggan, Brendan; Roca, Amancio; Zhang, Yong-Mei
Citation: Parnham, Stuart; Gaines, William; Duggan, Brendan; Marcotte, William; Hennig, Mirko. "NMR assignments of the N-terminal domain of Nephila clavipes spidroin 1." Biomol. NMR Assignments 5, 131-133 (2011).
Assembly members:
ApoAcpP-1, polymer, 79 residues, Formula weight is not available
Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
ApoAcpP-1: MDDIETRVRKLVAARFGVEE
CDIRLDSDFRNDFGAESLEV
VELVMALEAEFGVEIADDDA
ERIETVRQAIDYLEEAVPT
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 186 |
| 15N chemical shifts | 73 |
| 1H chemical shifts | 457 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ApoAcpP-1 | 1 |
Entities:
Entity 1, ApoAcpP-1 79 residues - Formula weight is not available
| 1 | MET | ASP | ASP | ILE | GLU | THR | ARG | VAL | ARG | LYS | ||||
| 2 | LEU | VAL | ALA | ALA | ARG | PHE | GLY | VAL | GLU | GLU | ||||
| 3 | CYS | ASP | ILE | ARG | LEU | ASP | SER | ASP | PHE | ARG | ||||
| 4 | ASN | ASP | PHE | GLY | ALA | GLU | SER | LEU | GLU | VAL | ||||
| 5 | VAL | GLU | LEU | VAL | MET | ALA | LEU | GLU | ALA | GLU | ||||
| 6 | PHE | GLY | VAL | GLU | ILE | ALA | ASP | ASP | ASP | ALA | ||||
| 7 | GLU | ARG | ILE | GLU | THR | VAL | ARG | GLN | ALA | ILE | ||||
| 8 | ASP | TYR | LEU | GLU | GLU | ALA | VAL | PRO | THR |
Samples:
15N-ApoAcpP-1: ApoAcpP-1, [U-99% 15N], 1.0 mM; potassium phosphate 20 mM; sodium chloride 50 mM; DTT 2 mM; D2O 10%; H2O 90%
13C15N-ApoAcpP-1: ApoAcpP-1, [U-99% 13C; U-99% 15N], 1.0 mM; potassium phosphate 20 mM; sodium chloride 50 mM; DTT 2 mM; D2O 10%; H2O 90%
sample_conditions_1: ionic strength: 70 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | 15N-ApoAcpP-1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | 13C15N-ApoAcpP-1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | 13C15N-ApoAcpP-1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | 13C15N-ApoAcpP-1 | isotropic | sample_conditions_1 |
| 3D HNCACB | 13C15N-ApoAcpP-1 | isotropic | sample_conditions_1 |
| 3D HNCO | 13C15N-ApoAcpP-1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | 13C15N-ApoAcpP-1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | 13C15N-ApoAcpP-1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | 13C15N-ApoAcpP-1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | 15N-ApoAcpP-1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | 13C15N-ApoAcpP-1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | 13C15N-ApoAcpP-1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.0.4, Bruker Biospin - collection, processing
CcpNMR v2.2.2, CCPN - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 850 MHz
Related Database Links:
| DBJ | BAK89639 BAP23701 BAP51301 BAQ40472 BAR68359 |
| EMBL | CAW28181 CCQ87170 CDH71178 CDH77747 CDI90080 |
| GB | AAC38778 AAG05258 AAT50654 ABJ11059 AEO75655 |
| REF | NP_250560 WP_003106681 WP_003108704 WP_003132735 WP_009876275 |
| SP | O52658 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts