BMRB Entry 18828
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR18828
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Title: NMR Assignment of Arabidopsis thaliana cytochrome c in its reduced state PubMed: 23438074
Deposition date: 2012-11-07 Original release date: 2014-02-14
Authors: Moreno-Beltran, Blas; Diaz-Moreno, Irene; Diaz-Quintana, Antonio; De la Rosa, Miguel
Citation: Hervas, Manuel; Bashir, Qamar; Leferink, Nicole; Ferreira, Patricia; Moreno-Beltran, Blas; Westphal, Adrie; Diaz-Moreno, Irene; Medina, Milagros; de la Rosa, Miguel; Ubbink, Marcellus; Navarro, Jose; van Berkel, Willem. "Communication between (L)-galactono-1,4-lactone dehydrogenase and cytochrome c." FEBS J. 280, 1830-1840 (2013).
Assembly members:
cytochrome_c_peptide, polymer, 111 residues, Formula weight is not available
HEME C, non-polymer, 618.503 Da.
Natural source: Common Name: thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
cytochrome_c_peptide: ASFDEAPPGNPKAGEKIFRT
KCAQCHTVEKGAGHKQGPNL
NGLFGRQSGTTPGYSYSAAN
KSMAVNWEEKTLYDYLLNPK
KYIPGTKMVFPGLKKPQDRA
DLIAYLKEGTA
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 112 |
| 1H chemical shifts | 122 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cytochrome c | 1 |
| 2 | HEC | 2 |
Entities:
Entity 1, cytochrome c 111 residues - Formula weight is not available
| 1 | ALA | SER | PHE | ASP | GLU | ALA | PRO | PRO | GLY | ASN | ||||
| 2 | PRO | LYS | ALA | GLY | GLU | LYS | ILE | PHE | ARG | THR | ||||
| 3 | LYS | CYS | ALA | GLN | CYS | HIS | THR | VAL | GLU | LYS | ||||
| 4 | GLY | ALA | GLY | HIS | LYS | GLN | GLY | PRO | ASN | LEU | ||||
| 5 | ASN | GLY | LEU | PHE | GLY | ARG | GLN | SER | GLY | THR | ||||
| 6 | THR | PRO | GLY | TYR | SER | TYR | SER | ALA | ALA | ASN | ||||
| 7 | LYS | SER | MET | ALA | VAL | ASN | TRP | GLU | GLU | LYS | ||||
| 8 | THR | LEU | TYR | ASP | TYR | LEU | LEU | ASN | PRO | LYS | ||||
| 9 | LYS | TYR | ILE | PRO | GLY | THR | LYS | MET | VAL | PHE | ||||
| 10 | PRO | GLY | LEU | LYS | LYS | PRO | GLN | ASP | ARG | ALA | ||||
| 11 | ASP | LEU | ILE | ALA | TYR | LEU | LYS | GLU | GLY | THR | ||||
| 12 | ALA |
Entity 2, HEC - C34 H34 Fe N4 O4 - 618.503 Da.
| 1 | HEC |
Samples:
AtCc_sample: polymer, [U-99% 15N], 2 mM; sodium phosphate 5 mM; H2O 90%; D2O 10%
AtCc_cond: ionic strength: 0.05 M; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | AtCc_sample | isotropic | AtCc_cond |
| 3D 1H-15N NOESY | AtCc_sample | isotropic | AtCc_cond |
| 3D 1H-15N TOCSY | AtCc_sample | isotropic | AtCc_cond |
Software:
SPARKY, Goddard - chemical shift assignment
TOPSPIN, Bruker Biospin - processing
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker Avance 700 MHz
Related Database Links:
| EMBL | CAB39628 CAB78127 CDX90744 |
| GB | AAK76618 AAL85104 AAM64617 AEE82832 EFH48744 |
| REF | NP_192742 XP_002872485 XP_006288926 XP_006397109 XP_009134179 |
| SP | Q9T0G2 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts