BMRB Entry 18884
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18884
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Title: CD79b cytosolic domain PubMed: 23667548
Deposition date: 2012-12-07 Original release date: 2013-10-15
Authors: Mayzel, Maxim
Citation: Isaksson, Linnea; Mayzel, Maxim; Saline, Maria; Pedersen, Anders; Brutscher, Bernhard; Karlsson, Goran; Orekhov, Vladislav. "Highly efficient NMR assignment of intrinsically disordered proteins: application to B- and T cell receptor domains" PLoS One 8, e62947-e62947 (2013).
Assembly members:
CD79b, polymer, 51 residues, 5702.0248 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: Wheat germ - cell freeE. coli - cell free
Entity Sequences (FASTA):
CD79b: SLLDKDDSKAGMEEDHTYEG
LDIDQTATYEDIVTLRTGEV
KWSVGEHPGQE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 145 |
| 15N chemical shifts | 48 |
| 1H chemical shifts | 48 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CD79b | 1 |
Entities:
Entity 1, CD79b 51 residues - 5702.0248 Da.
| 1 | SER | LEU | LEU | ASP | LYS | ASP | ASP | SER | LYS | ALA | ||||
| 2 | GLY | MET | GLU | GLU | ASP | HIS | THR | TYR | GLU | GLY | ||||
| 3 | LEU | ASP | ILE | ASP | GLN | THR | ALA | THR | TYR | GLU | ||||
| 4 | ASP | ILE | VAL | THR | LEU | ARG | THR | GLY | GLU | VAL | ||||
| 5 | LYS | TRP | SER | VAL | GLY | GLU | HIS | PRO | GLY | GLN | ||||
| 6 | GLU |
Samples:
Native: CD79b, [U-100% 13C; U-100% 15N], 0.1 mM; DTT 2.0 mM; EDTA 1.0 mM; potassiup phosphate 20.0 mM; azide 0.02%; Complete EDTA-free 1 x; H2O 90%; D2O 10%
1: ionic strength: 0.020 M; pH: 6.800; pressure: 1.000 atm; temperature: 298.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC/HMQC | Native | isotropic | 1 |
| 3D HN(CA)CO | Native | isotropic | 1 |
| 3D HNCA | Native | isotropic | 1 |
| 3D HNCACB | Native | isotropic | 1 |
| 3D HNCO | Native | isotropic | 1 |
| 3D HN(CO)CA | Native | isotropic | 1 |
| 3D HN(CO)CACB | Native | isotropic | 1 |
Software:
AutoAssign v2.3.0, Zimmerman, Moseley, Kulikowski and Montelione - Automatic assignment
ANALYSIS v2.2, CCPN - data analysis
MDDNMR v2.1, Swedish NMR Center, University of Gothenburg - Spectrum processing
TANSY v1.0, Swedish NMR Center, University of Gothenburg - Spectra processing & analysis
NMRPipe vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - Spectrum processing
NMR spectrometers:
- Varian UnityInova 600 MHz
Related Database Links:
| UNP | P40259 |
| BMRB | 19650 19651 |
| DBJ | BAA25652 BAD96674 BAD96930 BAG36353 |
| EMBL | CAA58522 |
| GB | AAA58387 AAA64459 AAA72424 AAB24822 AAC60654 |
| REF | NP_000617 NP_001035022 NP_001230841 NP_001248454 NP_067613 |
| SP | P40259 |
| TPG | DAA18410 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts