BMRB Entry 18895

Name: Backbone and partial sidechain assignment of the microtubule binding domain of the MAP1B light chain
Published: 2013-01-29

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18895

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Backbone and partial sidechain assignment of the microtubule binding domain of the MAP1B light chain PubMed: 23339032

Deposition date: 2012-12-13 Original release date: 2013-01-29

Authors: Orban-Nemeth, Zsuzsanna; Propst, Friedrich; Henen, Morkos; Konrat, Robert; Kozminski, Wiktor; Zerko, Szymon; Saxena, Saurabh; Stanek, Jan; Geist, Leonhard

Citation: Orban-Nemeth, Zsuzsanna; Henen, Morkos; Geist, Leonhard; Zerko, Szymon; Saxena, Saurabh; Stanek, Jan; Komiski, Wiktor; Propst, Friedrich; Konrat, Robert. "Backbone and partial side chain assignment of the microtubule binding domain of the MAP1B light chain." Biomol. NMR Assignments ., .-. (2013).

Assembly members:
MAP1B, polymer, 150 residues, Formula weight is not available

Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
MAP1B: MGSSHHHHHHSSGLVPRGSH MEFMVDPEALAIEQNLGKAL KKDLKEKAKTKKPGTKTKSS SPVKKGDGKSKPSAASPKPG ALKESSDKVSRVASPKKKES VEKAMKTTTTPEVKATRGEE KDKETKNAANASASKSVKTA TAGPGTTKTA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	489
15N chemical shifts	124
1H chemical shifts	757

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	N-terminal MAP1B LC	1

Entities:

Entity 1, N-terminal MAP1B LC 150 residues - Formula weight is not available

1	MET	GLY	SER	SER	HIS	HIS	HIS	HIS	HIS	HIS
2	SER	SER	GLY	LEU	VAL	PRO	ARG	GLY	SER	HIS
3	MET	GLU	PHE	MET	VAL	ASP	PRO	GLU	ALA	LEU
4	ALA	ILE	GLU	GLN	ASN	LEU	GLY	LYS	ALA	LEU
5	LYS	LYS	ASP	LEU	LYS	GLU	LYS	ALA	LYS	THR
6	LYS	LYS	PRO	GLY	THR	LYS	THR	LYS	SER	SER
7	SER	PRO	VAL	LYS	LYS	GLY	ASP	GLY	LYS	SER
8	LYS	PRO	SER	ALA	ALA	SER	PRO	LYS	PRO	GLY
9	ALA	LEU	LYS	GLU	SER	SER	ASP	LYS	VAL	SER
10	ARG	VAL	ALA	SER	PRO	LYS	LYS	LYS	GLU	SER
11	VAL	GLU	LYS	ALA	MET	LYS	THR	THR	THR	THR
12	PRO	GLU	VAL	LYS	ALA	THR	ARG	GLY	GLU	GLU
13	LYS	ASP	LYS	GLU	THR	LYS	ASN	ALA	ALA	ASN
14	ALA	SER	ALA	SER	LYS	SER	VAL	LYS	THR	ALA
15	THR	ALA	GLY	PRO	GLY	THR	THR	LYS	THR	ALA

Samples:

sample_1: MAP1B, [U-95% 13C; U-90% 15N], 0.6 – 1 mM; D2O, [U-100% 2H], 10%; sodium chloride 300 mM; sodium phosphate 50 mM

sample_conditions_1: pH: 5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
5D HN(CA)CONH	sample_1	isotropic	sample_conditions_1
5D (HACA)CON(CA)CONH	sample_1	isotropic	sample_conditions_1
5D (H)NCO(NCA)CONH	sample_1	isotropic	sample_conditions_1
4D HNCACO	sample_1	isotropic	sample_conditions_1
5D HabCabCONH	sample_1	isotropic	sample_conditions_1
5D H(CC-tocsy)CONH	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - data analysis

MFT, Wiktor Kozminski - processing

TSAR, Kozminski, Zawadzka, Kazimiurcuk - chemical shift assignment

NMR spectrometers:

Agilent Direct Drive 700 MHz

RefSeq	NP_062090.1
EMBL	CAA34620 CAC16162
GB	EDM10176
PIR	S06017
REF	NP_062090 XP_008758883
SP	P15205

Biological Magnetic Resonance Data Bank