BMRB Entry 18949
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18949
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Title: The H/D-exchange Kinetics of the Escherichia coli Co-chaperonin GroES studied by 2D-NMR and DMSO-Quenched Exchange Methods PubMed: 23583779
Deposition date: 2013-01-14 Original release date: 2013-06-04
Authors: Chandak, Mahesh; Nakamura, Takashi; Makabe, Koki; Takenaka, Toshio; Mukaiyama, Atsushi; Chaudhuri, Tapan; Chen, Jin; Kato, Koichi; Kuwajima, Kunihiro
Citation: Chandak, Mahesh; Nakamura, Takashi; Makabe, Koki; Takenaka, Toshio; Mukaiyama, Atsushi; Chaudhuri, Tapan; Kato, Koichi; Kuwajima, Kunihiro. "The H/D-Exchange Kinetics of the Escherichia coli Co-Chaperonin GroES Studied by 2D NMR and DMSO-Quenched Exchange Methods." J. Mol. Biol. 425, 2541-2560 (2013).
Assembly members:
GroES, polymer, 97 residues, Formula weight is not available
Natural source: Common Name: E. Coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
GroES: MNIRPLHDRVIVLRLEVETK
SAGGIVLTGSAAAKSTRGEV
LAVGNGRILENGEVLPLDVL
VGDIVIFNDGYGVLSELIDN
EEVDIMSESDILAIVEA
- assigned_chemical_shifts
Data type | Count |
15N chemical shifts | 43 |
1H chemical shifts | 45 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | GroES_1 | 1 |
2 | GroES_2 | 1 |
3 | GroES_3 | 1 |
Entities:
Entity 1, GroES_1 97 residues - Formula weight is not available
1 | MET | ASN | ILE | ARG | PRO | LEU | HIS | ASP | ARG | VAL | ||||
2 | ILE | VAL | LEU | ARG | LEU | GLU | VAL | GLU | THR | LYS | ||||
3 | SER | ALA | GLY | GLY | ILE | VAL | LEU | THR | GLY | SER | ||||
4 | ALA | ALA | ALA | LYS | SER | THR | ARG | GLY | GLU | VAL | ||||
5 | LEU | ALA | VAL | GLY | ASN | GLY | ARG | ILE | LEU | GLU | ||||
6 | ASN | GLY | GLU | VAL | LEU | PRO | LEU | ASP | VAL | LEU | ||||
7 | VAL | GLY | ASP | ILE | VAL | ILE | PHE | ASN | ASP | GLY | ||||
8 | TYR | GLY | VAL | LEU | SER | GLU | LEU | ILE | ASP | ASN | ||||
9 | GLU | GLU | VAL | ASP | ILE | MET | SER | GLU | SER | ASP | ||||
10 | ILE | LEU | ALA | ILE | VAL | GLU | ALA |
Samples:
sample_1: DMSO, [U-100% 15N], 300 uM; D2O 95%; H2O 5%
sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 273 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | not available | isotropic | sample_conditions_1 |
Software:
NMRView, One Moon Scientific,INC (NIH) - chemical shift assignment
NMR spectrometers:
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts