BMRB Entry 18961
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18961
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Title: NMR assignments of a hypothetical pseudo-knotted protein HP0242 from H. pylori PubMed: 23824732
Deposition date: 2013-01-18 Original release date: 2014-02-14
Authors: Chien, Chih-Ta Henry; Wang, Lian-Wei; Liu, Yu-Nan; Lyu, Ping-Chiang; Hsu, Shang-Te Danny
Citation: Chien, Chih-Ta Henry; Wang, Liang-Wei; Liu, Yu-Nan; Hsu, Ban-Dar; Lyu, Ping-Chiang; Hsu, Shang-Te Danny. "NMR assignments of a hypothetical pseudo-knotted protein HP0242 from Helicobacter pylori." Biomol. NMR Assignments ., .-. (2013).
Assembly members:
HP0242, polymer, 94 residues, Formula weight is not available
Natural source: Common Name: Helicobacter pylori Taxonomy ID: 210 Superkingdom: Bacteria Kingdom: not available Genus/species: Helicobacter pylori
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
HP0242: MRDYSELEIFEGNPLDKWND
IIFHASKKLSKKELERLLEL
LALLETFIEKEDLEEKFESF
AKALRIDEELQQKIESRKTD
IVIQSMANILSGNE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 419 |
| 15N chemical shifts | 91 |
| 1H chemical shifts | 639 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HP0242 | 1 |
Entities:
Entity 1, HP0242 94 residues - Formula weight is not available
| 1 | MET | ARG | ASP | TYR | SER | GLU | LEU | GLU | ILE | PHE | ||||
| 2 | GLU | GLY | ASN | PRO | LEU | ASP | LYS | TRP | ASN | ASP | ||||
| 3 | ILE | ILE | PHE | HIS | ALA | SER | LYS | LYS | LEU | SER | ||||
| 4 | LYS | LYS | GLU | LEU | GLU | ARG | LEU | LEU | GLU | LEU | ||||
| 5 | LEU | ALA | LEU | LEU | GLU | THR | PHE | ILE | GLU | LYS | ||||
| 6 | GLU | ASP | LEU | GLU | GLU | LYS | PHE | GLU | SER | PHE | ||||
| 7 | ALA | LYS | ALA | LEU | ARG | ILE | ASP | GLU | GLU | LEU | ||||
| 8 | GLN | GLN | LYS | ILE | GLU | SER | ARG | LYS | THR | ASP | ||||
| 9 | ILE | VAL | ILE | GLN | SER | MET | ALA | ASN | ILE | LEU | ||||
| 10 | SER | GLY | ASN | GLU |
Samples:
sample_1: HP0242, [U-100% 13C; U-100% 15N], 818 uM; H2O 90%; D2O 10%
sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCACO | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection
SPARKY, Goddard - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAJ54847 BAJ57150 BAJ57834 BAJ59335 BAM96160 |
| EMBL | CAX28791 CBI66878 |
| GB | AAD05799 AAD07312 ABF84312 ACD47706 ACJ07401 |
| REF | NP_207040 WP_001206042 WP_001206043 WP_001206044 WP_001206045 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts