BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18986

Title: 1H, 13C, and 15N Chemical Shift Assignments for the C-terminus of the minichromosome maintenance protein MCM from Sulfolobus solfataricus   PubMed: 23934138

Deposition date: 2013-01-29 Original release date: 2013-09-30

Authors: Wiedemann, Christoph; Ohlenschlager, Oliver; Medagli, Barbara; Onesti, Silvia; Gorlach, Matthias

Citation: Wiedemann, Christoph; Ohlenschlager, Oliver; Medagli, Barbara; Onesti, Silvia; Gorlach, Matthias. "(1)H, (15)N and (13)C chemical shift assignments for the winged helix domains of two archeal MCM C-termini."  Biomol. NMR Assignments ., .-. (2013).

Assembly members:
C-terminus_of_the_minichromosome_maintenance_protein_MCM, polymer, 87 residues, 9674.474 Da.

Natural source:   Common Name: Sulfolobus solfataricus   Taxonomy ID: 2287   Superkingdom: Archaea   Kingdom: not available   Genus/species: Sulfolobus solfataricus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
C-terminus_of_the_minichromosome_maintenance_protein_MCM: GSHMGESGKIDIDTIMTGKP KSAREKMMKIIEIIDSLAVS SECAKVKDILKEAQQVGIEK SNIEKLLTDMRKSGIIYEAK PECYKKV

Data sets:
Data typeCount
13C chemical shifts391
15N chemical shifts82
1H chemical shifts637

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C-terminus of the minichromosome maintenance protein MCM1

Entities:

Entity 1, C-terminus of the minichromosome maintenance protein MCM 87 residues - 9674.474 Da.

1   GLYSERHISMETGLYGLUSERGLYLYSILE
2   ASPILEASPTHRILEMETTHRGLYLYSPRO
3   LYSSERALAARGGLULYSMETMETLYSILE
4   ILEGLUILEILEASPSERLEUALAVALSER
5   SERGLUCYSALALYSVALLYSASPILELEU
6   LYSGLUALAGLNGLNVALGLYILEGLULYS
7   SERASNILEGLULYSLEULEUTHRASPMET
8   ARGLYSSERGLYILEILETYRGLUALALYS
9   PROGLUCYSTYRLYSLYSVAL

Samples:

sample_1: C-terminus of the minichromosome maintenance protein MCM, [U-100% 13C; U-100% 15N], 700 uM; potassium phosphate 10 mM; sodium chloride 150 mM; sodium azide 0.05 w/v

sample_2: C-terminus of the minichromosome maintenance protein MCM, [U-100% 13C; U-100% 15N], 700 uM; potassium phosphate 10 mM; sodium chloride 150 mM; sodium azide 0.05 w/v

sample_conditions_1: ionic strength: 160 mM; pH: 6.2; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_2isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_2isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_2isotropicsample_conditions_1

Software:

CYANA v3, Guntert, Mumenthaler and Wuthrich - structure solution

TOPSPIN, Bruker Biospin - collection & processing

CcpNMR, CCPN - data analysis

OPAL, Luginbuhl, Guntert, Billeter and Wuthrich - refinement

NMR spectrometers:

  • Bruker AvanceIII 600 MHz

Related Database Links:

UniProtKB Q9UXG1
PDB
EMBL CAB57529
GB AAK41071 ACP35470 ACP38121 ACP45628 ACP48570
REF WP_009991364 WP_012711366 WP_012713703 WP_012716161 WP_012717457
SP Q9UXG1

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts