BMRB Entry 19061
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19061
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Title: 1H,13C and 15N resonance assignments of Ca2+ - bound human S100A15 PubMed: 22392334
Deposition date: 2013-02-27 Original release date: 2015-07-24
Authors: Cho, Ching Chang; Yu, Chin
Citation: Penumutchu, Srinivasa; Mohan, Sepuru; Yu, Chin. "(1)H, (15)N and (13)C assignments of the calcium bound S100P." Biomol. NMR Assignments 7, 5-8 (2013).
Assembly members:
S100A15, polymer, 92 residues, 10696.9961 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
S100A15: MTKLEEHLEGIVNIFHQYSS
RVGHFDTLSKGELKQLLTKE
LANTIKNIKDKAVIDEIFQG
LDANQDEQVTFQEFISLVAI
ALKAAHYHTHKE
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 305 |
15N chemical shifts | 76 |
1H chemical shifts | 472 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | S100A15 | 1 |
Entities:
Entity 1, S100A15 92 residues - 10696.9961 Da.
1 | MET | THR | LYS | LEU | GLU | GLU | HIS | LEU | GLU | GLY | ||||
2 | ILE | VAL | ASN | ILE | PHE | HIS | GLN | TYR | SER | SER | ||||
3 | ARG | VAL | GLY | HIS | PHE | ASP | THR | LEU | SER | LYS | ||||
4 | GLY | GLU | LEU | LYS | GLN | LEU | LEU | THR | LYS | GLU | ||||
5 | LEU | ALA | ASN | THR | ILE | LYS | ASN | ILE | LYS | ASP | ||||
6 | LYS | ALA | VAL | ILE | ASP | GLU | ILE | PHE | GLN | GLY | ||||
7 | LEU | ASP | ALA | ASN | GLN | ASP | GLU | GLN | VAL | THR | ||||
8 | PHE | GLN | GLU | PHE | ILE | SER | LEU | VAL | ALA | ILE | ||||
9 | ALA | LEU | LYS | ALA | ALA | HIS | TYR | HIS | THR | HIS | ||||
10 | LYS | GLU |
Samples:
sample_1: S100A15, [U-100% 13C; U-100% 15N], 1.0 mM; TRIS 10 mM; sodium chloride 100 mM; Calcium chloride 10 mM; sodium azide 0.1 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.1 M; pH: 6.2; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HCCH-COSY | sample_1 | isotropic | sample_conditions_1 |
3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMR, Varian - collection, processing
SPARKY, Goddard - chemical shift assignment, data analysis
NMR spectrometers:
- Varian VNMR 700 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts