BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19114

Title: 4E-BP2   PubMed: 24207126

Deposition date: 2013-03-27 Original release date: 2014-02-12

Authors: Forman-Kay, Julie; Bah, Alaji

Citation: Lukhele, Sabelo; Bah, Alaji; Lin, Hong; Sonenberg, Nahum; Forman-Kay, Julie. "Interaction of the eukaryotic initiation factor 4E with 4E-BP2 at a dynamic bipartite interface."  Structure 21, 2186-2196 (2013).

Assembly members:
4E-BP2, polymer, 128 residues, 13798.3 Da.

Natural source:   Common Name: Humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
4E-BP2: MPLGSPEFMSSSAGSGHQPS QSRAIPTRTVAISDAAQLPH DYCTTPGGTLFSTTPGGTRI IYDRKFLLDRRNSPMAQTPP CHLPNIPGVTSPGTLIEDSK VEVNNLDNLDNHDRKHAVGD DAQFEMDI

Data sets:
Data typeCount
13C chemical shifts438
15N chemical shifts113
1H chemical shifts209

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
14E-BP2, native1
24E-BP2, intrinsically disordered1

Entities:

Entity 1, 4E-BP2, native 128 residues - 13798.3 Da.

Residues -6 to 0 is a tag remaining after cleaving of GST fusion protein.

1   METPROLEUGLYSERPROGLUPHEMETSER
2   SERSERALAGLYSERGLYHISGLNPROSER
3   GLNSERARGALAILEPROTHRARGTHRVAL
4   ALAILESERASPALAALAGLNLEUPROHIS
5   ASPTYRCYSTHRTHRPROGLYGLYTHRLEU
6   PHESERTHRTHRPROGLYGLYTHRARGILE
7   ILETYRASPARGLYSPHELEULEUASPARG
8   ARGASNSERPROMETALAGLNTHRPROPRO
9   CYSHISLEUPROASNILEPROGLYVALTHR
10   SERPROGLYTHRLEUILEGLUASPSERLYS
11   VALGLUVALASNASNLEUASPASNLEUASP
12   ASNHISASPARGLYSHISALAVALGLYASP
13   ASPALAGLNPHEGLUMETASPILE

Samples:

sample_1: 4E-BP2, [U-99% 13C; U-99% 15N], 800 – 1200 uM; DTT 2 mM; sodium chloride 100 mM; sodium phosphate 30 mM; EDTA 1 mM; benzamidine 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 150 mM; pH: 6.0; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

BMRB 19905
DBJ BAG11409 BAG36466
EMBL CAG47036
GB AAA62270 AAH05057 AAH50633 AAP35981 AAX32712
PRF 2021415B
REF NP_001178078 NP_001244839 NP_004087 XP_002718482 XP_003133106
SP Q13542
TPG DAA14291

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts