BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules 		Member of WWPDB

BMRB Entry 19161

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19161

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Title: Backbone chemical shift assignment of porcine amelogenin in the presence of SDS micelles   PubMed: 24114119

Deposition date: 2013-04-12 Original release date: 2014-02-12

Authors: Dutta, Kaushik; Evans, John; Moradian-Oldak, Janet

Citation: Chandrababu, Karthik Balakrishna; Dutta, Kaushik; Lokappa, Sowmya Bekshe; Ndao, Moise; Evans, John Spencer; Moradian-Oldak, Janet. "Structural adaptation of tooth enamel protein amelogenin in the presence of SDS micelles."  Biopolymers 101, 525-535 (2014).

Assembly members:
Amelogenin, polymer, 173 residues, Formula weight is not available

Natural source:   Common Name: Porcine   Taxonomy ID: 9823   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Sus scrofa

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Amelogenin: MPLPPHPGHPGYINFSYEVL TPLKWYQNMIRHPYTSYGYE PMGGWLHHQIIPVVSQQTPQ SHALQPHHHIPMVPAQQPGI PQQPMMPLPGQHSMTPTQHH QPNLPLPAQQPFQPQPVQPQ PHQPLQPQSPMHPIQPLLPQ PPLPPMFSMQSLLPDLPLEA WPATDKTKREEVD

Data sets:
Data typeCount
13C chemical shifts369
15N chemical shifts100
1H chemical shifts100

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Amelogenin1

Entities:

Entity 1, Amelogenin 173 residues - Formula weight is not available

1   METPROLEUPROPROHISPROGLYHISPRO
2   GLYTYRILEASNPHESERTYRGLUVALLEU
3   THRPROLEULYSTRPTYRGLNASNMETILE
4   ARGHISPROTYRTHRSERTYRGLYTYRGLU
5   PROMETGLYGLYTRPLEUHISHISGLNILE
6   ILEPROVALVALSERGLNGLNTHRPROGLN
7   SERHISALALEUGLNPROHISHISHISILE
8   PROMETVALPROALAGLNGLNPROGLYILE
9   PROGLNGLNPROMETMETPROLEUPROGLY
10   GLNHISSERMETTHRPROTHRGLNHISHIS
11   GLNPROASNLEUPROLEUPROALAGLNGLN
12   PROPHEGLNPROGLNPROVALGLNPROGLN
13   PROHISGLNPROLEUGLNPROGLNSERPRO
14   METHISPROILEGLNPROLEULEUPROGLN
15   PROPROLEUPROPROMETPHESERMETGLN
16   SERLEULEUPROASPLEUPROLEUGLUALA
17   TRPPROALATHRASPLYSTHRLYSARGGLU
18   GLUVALASP

Samples:

sample_1: Amelogenin, [U-13C; U-15N; U-2H], 75 uM; H2O, Natural Abundance, 90%; D2O, Natural Abundance, 10%

sample_2: Amelogenin, [U-13C; U-15N; U-2H], 75 uM; SDS, [U-2H], 100 mM; H2O, Natural Abundance, 90%; D2O, Natural Abundance, 10%

sample_conditions_1: ionic strength: 0 M; pH: 3.8; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
TROSYsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HN(CA)COsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HN(CO)CACBsample_2isotropicsample_conditions_1

Software:

NMRView v9, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

GB AAB30102 AAB30103 AAB41110 AAB41111 ABY48722
REF NP_998965 NP_999071
SP P45561

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts