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Biological Magnetic Resonance Data Bank


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BMRB Entry 19164

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19164
MolProbity Validation Chart

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Title: solution structure of cerebral dopamine neurotrophic factor (CDNF)   PubMed: 22528768

Deposition date: 2013-04-15 Original release date: 2013-08-15

Authors: Latge, C.; Cabral, K.; Raymundo, D.; Foguel, D.; Herrmann, T.; Almeida, Marcius; Al, M.

Citation: Latge, Cristiane; Cabral, Katia; Almeida, Marcius; Foguel, Debora. "(1)H-, (13)C- and (15)N-NMR assignment of the N-terminal domain of human cerebral dopamine neurotrophic factor (CDNF)."  Biomol. NMR Assignments 7, 101-103 (2013).

Assembly members:
CEREBRAL_DOPAMINE_NEUROTROPHIC_FACTOR, polymer, 161 residues, 18318.1787 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: escherichia coli

Entity Sequences (FASTA):
CEREBRAL_DOPAMINE_NEUROTROPHIC_FACTOR: QEAGGRPGADCEVCKEFLNR FYKSLIDRGVNFSLDTIEKE LISFCLDTKGKENRLCYYLG ATKDAATKILSEVTRPMSVH MPAMKICEKLKKLDSQICEL KYEKTLDLASVDLRKMRVAE LKQILHSWGEECRACAEKTD YVNLIQELAPKYAATHPKTE L

Data sets:
Data typeCount
13C chemical shifts709
15N chemical shifts149
1H chemical shifts1156
T1 relaxation values81
T2 relaxation values81
heteronuclear NOE values122

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CEREBRAL DOPAMINE NEUROTROPHIC FACTOR1

Entities:

Entity 1, CEREBRAL DOPAMINE NEUROTROPHIC FACTOR 161 residues - 18318.1787 Da.

1   GLNGLUALAGLYGLYARGPROGLYALAASP
2   CYSGLUVALCYSLYSGLUPHELEUASNARG
3   PHETYRLYSSERLEUILEASPARGGLYVAL
4   ASNPHESERLEUASPTHRILEGLULYSGLU
5   LEUILESERPHECYSLEUASPTHRLYSGLY
6   LYSGLUASNARGLEUCYSTYRTYRLEUGLY
7   ALATHRLYSASPALAALATHRLYSILELEU
8   SERGLUVALTHRARGPROMETSERVALHIS
9   METPROALAMETLYSILECYSGLULYSLEU
10   LYSLYSLEUASPSERGLNILECYSGLULEU
11   LYSTYRGLULYSTHRLEUASPLEUALASER
12   VALASPLEUARGLYSMETARGVALALAGLU
13   LEULYSGLNILELEUHISSERTRPGLYGLU
14   GLUCYSARGALACYSALAGLULYSTHRASP
15   TYRVALASNLEUILEGLNGLULEUALAPRO
16   LYSTYRALAALATHRHISPROLYSTHRGLU
17   LEU

Samples:

sample_1: CEREBRAL DOPAMINE NEUROTROPHIC FACTOR, [U-13C; U-15N], 0.7 mM; MES 20 mM; NaCl 150 mM; NaN3 5 mM

sample_2: CEREBRAL DOPAMINE NEUROTROPHIC FACTOR, [U-13C; U-15N], 0.65 mM; Sodium Phosphate 20 mM; NaCl 100 mM; NaN3 3 mM; EDTA 1 mM

sample_conditions_1: ionic strength: 170.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

sample_conditions_2: ionic strength: 120.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_2solutionsample_conditions_2
NOESYsample_2solutionsample_conditions_2
TOCSYsample_1solutionsample_conditions_1
HSQCsample_1solutionsample_conditions_1
COSYsample_1solutionsample_conditions_1
HNCACBsample_1solutionsample_conditions_1
CBCACONHsample_1solutionsample_conditions_1
HBHACONHsample_1solutionsample_conditions_1
HNCOsample_1solutionsample_conditions_1
HSQCsample_1solutionsample_conditions_1
TOCSYsample_1solutionsample_conditions_1
HSQCsample_1solutionsample_conditions_1

Software:

AutoDep v4.3 - collection

UNIO v10 - chemical shift assignment

CNS v1.3, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 1000 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

UNP CDNF_HUMAN

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts