BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19236

Title: Backbone (1H, 15N, CA, CB) assignments of D. crassirhizoma Plastocyanin (Zn)

Deposition date: 2013-05-13 Original release date: 2013-06-10

Authors: Guan, Jia-Ying; Ubbink, Marcellus

Citation: Guan, Jia-Ying; Foester, Johannes; Drijfhout, Jan Wouter; Timmer, Monika; Blok, Anneloes; Ullmann, G. Matthias; Ubbink, Marcellus. "Dynamics in the complex of plastocyanin and tetralysine peptides"  Not known ., .-..

Assembly members:
plastocyanin, polymer, 102 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Ferns   Taxonomy ID: 97234   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Dryopteris crassirhizoma

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
plastocyanin: AKVEVGDEVGNFKFYPDSIT VSAGEAVEFTLVGETGHNIV FDIPAGAPGTVASELKAASM DENDLLSEDEPSFKAKVSTP GTYTFYCTPHKSANMKGTLT VK

Data sets:
Data typeCount
13C chemical shifts195
15N chemical shifts94
1H chemical shifts94

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1plastocyanin1
2zinc2

Entities:

Entity 1, plastocyanin 102 residues - Formula weight is not available

1   ALALYSVALGLUVALGLYASPGLUVALGLY
2   ASNPHELYSPHETYRPROASPSERILETHR
3   VALSERALAGLYGLUALAVALGLUPHETHR
4   LEUVALGLYGLUTHRGLYHISASNILEVAL
5   PHEASPILEPROALAGLYALAPROGLYTHR
6   VALALASERGLULEULYSALAALASERMET
7   ASPGLUASNASPLEULEUSERGLUASPGLU
8   PROSERPHELYSALALYSVALSERTHRPRO
9   GLYTHRTYRTHRPHETYRCYSTHRPROHIS
10   LYSSERALAASNMETLYSGLYTHRLEUTHR
11   VALLYS

Entity 2, zinc - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: plastocyanin, [U-13C; U-15N], 2.4 ± 0.1 mM; sodium phosphate 10 mM

sample_conditions_1: ionic strength: 10 mM; pH: 6.5; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
SP Q7SIB8

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts