BMRB Entry 19252
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19252
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Title: RRM domain of HUMAN RBM7
Deposition date: 2013-05-21 Original release date: 2015-01-26
Authors: Sikorsky, Tomas; Hrossova, Dominika; Vanacova, Stepanka; Stefl, Richard
Citation: Sikorsky, Tomas; Stefl, Richard; Hrossova, Dominika; Vanacova, Stepanka. "RNA binding properties of the human NEXT complex" Not known ., .-..
Assembly members:
RBM7, polymer, 101 residues, 11473.301 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
RBM7: GSEFELRRQQALAEADRTLF
VGNLETKVTEELLFELFHQA
GPVIKVKIPKDKDGKPKQFA
FVNFKHEVSVPYAMNLLNGI
KLYGRPIKIQFRSGSSHAPQ
D
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 298 |
15N chemical shifts | 80 |
1H chemical shifts | 602 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | RRM domain of HUMAN RBM7 | 1 |
Entities:
Entity 1, RRM domain of HUMAN RBM7 101 residues - 11473.301 Da.
1 | GLY | SER | GLU | PHE | GLU | LEU | ARG | ARG | GLN | GLN | ||||
2 | ALA | LEU | ALA | GLU | ALA | ASP | ARG | THR | LEU | PHE | ||||
3 | VAL | GLY | ASN | LEU | GLU | THR | LYS | VAL | THR | GLU | ||||
4 | GLU | LEU | LEU | PHE | GLU | LEU | PHE | HIS | GLN | ALA | ||||
5 | GLY | PRO | VAL | ILE | LYS | VAL | LYS | ILE | PRO | LYS | ||||
6 | ASP | LYS | ASP | GLY | LYS | PRO | LYS | GLN | PHE | ALA | ||||
7 | PHE | VAL | ASN | PHE | LYS | HIS | GLU | VAL | SER | VAL | ||||
8 | PRO | TYR | ALA | MET | ASN | LEU | LEU | ASN | GLY | ILE | ||||
9 | LYS | LEU | TYR | GLY | ARG | PRO | ILE | LYS | ILE | GLN | ||||
10 | PHE | ARG | SER | GLY | SER | SER | HIS | ALA | PRO | GLN | ||||
11 | ASP |
Samples:
sample_1: RBM7, [U-99% 13C; U-99% 15N], 0.3 mM; BISTRIS 20 mM; beta-mercaptoethanol 4 mM; sodium chloride 200 mM
sample_conditions_1: ionic strength: 200 mM; pH: 7; pressure: 1 atm; temperature: 293.15 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA, Keller and Wuthrich - chemical shift assignment
NMR spectrometers:
- Bruker Avance 700 MHz
- Bruker Avance 950 MHz
Related Database Links:
PDB | |
DBJ | BAA92036 BAD92990 BAG35505 BAG73694 |
EMBL | CAH93091 |
GB | AAD39257 AAH34381 AAH70262 ADQ31580 AIC50533 |
REF | NP_001248000 NP_001272974 NP_057174 XP_002754472 XP_002822554 |
SP | Q9Y580 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts