BMRB Entry 19285
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19285
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Title: Optimized Ratiometric Calcium Sensors For Functional In Vivo Imaging of Neurons and T-Lymphocytes PubMed: 24390440
Deposition date: 2013-06-04 Original release date: 2014-01-02
Authors: Russo, Luigi; Becker, Stefan; Griesinger, Christian
Citation: Thestrup, Thomas; Litzlbauer, Julia; Bartholomaus, Ingo; Mues, Marsilius; Russo, Luigi; Dana, Hod; Kovalchuk, Yuri; Liang, Yajie; Kalamakis, Georgios; Laukat, Yvonne; Becker, Stefan; Witte, Gregor; Geiger, Anselm; Allen, Taylor; Rome, Lawrence; Chen, Tsai-Wen; Kim, Douglas; Garaschuk, Olga; Griesinger, Christian; Griesbeck, Oliver. "Optimized ratiometric calcium sensors for functional in vivo imaging of neurons and T lymphocytes." Nat. Methods 11, 175-182 (2014).
Assembly members:
entity, polymer, 69 residues, 8066.708 Da.
Natural source: Common Name: oyster toadfish Taxonomy ID: 8068 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Opsanus tau
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: SEEELSECFRIFDKDGNGFI
DREEFGDIIRLTGEQLTDED
VDEIFGDSDTDKNGRIDFDE
FLKMMENVQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 199 |
| 15N chemical shifts | 69 |
| 1H chemical shifts | 143 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Calcium Sensor | 1 |
Entities:
Entity 1, Calcium Sensor 69 residues - 8066.708 Da.
| 1 | SER | GLU | GLU | GLU | LEU | SER | GLU | CYS | PHE | ARG | ||||
| 2 | ILE | PHE | ASP | LYS | ASP | GLY | ASN | GLY | PHE | ILE | ||||
| 3 | ASP | ARG | GLU | GLU | PHE | GLY | ASP | ILE | ILE | ARG | ||||
| 4 | LEU | THR | GLY | GLU | GLN | LEU | THR | ASP | GLU | ASP | ||||
| 5 | VAL | ASP | GLU | ILE | PHE | GLY | ASP | SER | ASP | THR | ||||
| 6 | ASP | LYS | ASN | GLY | ARG | ILE | ASP | PHE | ASP | GLU | ||||
| 7 | PHE | LEU | LYS | MET | MET | GLU | ASN | VAL | GLN |
Samples:
sample_1: Calcium sensor, [U-13C; U-15N], ; H2O 90%; D2O 10%; TRIS 1 mM
sample_conditions_1: ionic strength: 0.1 M; pH: 7; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
Software:
CS-Rosetta, Shen, Vernon, Baker and Bax - structure solution
NMR spectrometers:
- Bruker DRX 700 MHz
- Bruker DRX 900 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts