BMRB Entry 19325
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19325
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Title: 1H, 13C, and 15N Chemical Shift Assignments for a proteasome related subunit PubMed: 24037519
Deposition date: 2013-06-27 Original release date: 2014-02-13
Authors: Wu, Yujie; Hu, Yunfei; Jin, Changwen
Citation: Wu, Yujie; Hu, Yunfei; Jin, Changwen. "(1)H, (13)C and (15)N resonance assignments of the VWA domain of Saccharomyces cerevisiae Rpn10, a regulatory subunit of 26S proteasome." Biomol. NMR Assignments ., .-. (2013).
Assembly members:
26s_proteasome_subunit, polymer, 207 residues, Formula weight is not available
Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
26s_proteasome_subunit: MVLEATVLVIDNSEYSRNGD
FPRTRFEAQIDSVEFIFQAK
RNSNPENTVGLISGAGANPR
VLSTFTAEFGKILAGLHDTQ
IEGKLHMATALQIAQLTLKH
RQNKVQHQRIVAFVCSPISD
SRDELIRLAKTLKKNNVAVD
IINFGEIEQNTELLDEFIAA
VNNPQEETSHLLTVTPGPRL
LYENIASSPIILEEGSSGMG
AFGGSGG
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 811 |
15N chemical shifts | 208 |
1H chemical shifts | 1332 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | 26S proteasome subunit monomer | 1 |
Entities:
Entity 1, 26S proteasome subunit monomer 207 residues - Formula weight is not available
1 | MET | VAL | LEU | GLU | ALA | THR | VAL | LEU | VAL | ILE | ||||
2 | ASP | ASN | SER | GLU | TYR | SER | ARG | ASN | GLY | ASP | ||||
3 | PHE | PRO | ARG | THR | ARG | PHE | GLU | ALA | GLN | ILE | ||||
4 | ASP | SER | VAL | GLU | PHE | ILE | PHE | GLN | ALA | LYS | ||||
5 | ARG | ASN | SER | ASN | PRO | GLU | ASN | THR | VAL | GLY | ||||
6 | LEU | ILE | SER | GLY | ALA | GLY | ALA | ASN | PRO | ARG | ||||
7 | VAL | LEU | SER | THR | PHE | THR | ALA | GLU | PHE | GLY | ||||
8 | LYS | ILE | LEU | ALA | GLY | LEU | HIS | ASP | THR | GLN | ||||
9 | ILE | GLU | GLY | LYS | LEU | HIS | MET | ALA | THR | ALA | ||||
10 | LEU | GLN | ILE | ALA | GLN | LEU | THR | LEU | LYS | HIS | ||||
11 | ARG | GLN | ASN | LYS | VAL | GLN | HIS | GLN | ARG | ILE | ||||
12 | VAL | ALA | PHE | VAL | CYS | SER | PRO | ILE | SER | ASP | ||||
13 | SER | ARG | ASP | GLU | LEU | ILE | ARG | LEU | ALA | LYS | ||||
14 | THR | LEU | LYS | LYS | ASN | ASN | VAL | ALA | VAL | ASP | ||||
15 | ILE | ILE | ASN | PHE | GLY | GLU | ILE | GLU | GLN | ASN | ||||
16 | THR | GLU | LEU | LEU | ASP | GLU | PHE | ILE | ALA | ALA | ||||
17 | VAL | ASN | ASN | PRO | GLN | GLU | GLU | THR | SER | HIS | ||||
18 | LEU | LEU | THR | VAL | THR | PRO | GLY | PRO | ARG | LEU | ||||
19 | LEU | TYR | GLU | ASN | ILE | ALA | SER | SER | PRO | ILE | ||||
20 | ILE | LEU | GLU | GLU | GLY | SER | SER | GLY | MET | GLY | ||||
21 | ALA | PHE | GLY | GLY | SER | GLY | GLY |
Samples:
sample_1: 26s_proteasome_subunit, [U-100% 13C; U-100% 15N], 1.0 mM; MES 30 mM; DTT 10 mM; sodium chloride 50 mM; DSS 0.03%; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.08 M; pH: 6.8; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
3D HCCH-COSY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Zhengrong and Bax - processing
NMRView, Johnson, One Moon Scientific - data analysis
CARA, Keller and Wuthrich - data analysis
NMR spectrometers:
- Bruker Avance 500 MHz
- Bruker Avance 800 MHz
Related Database Links:
PDB | |
DBJ | BAA11207 GAA23917 |
EMBL | CAY80217 |
GB | AAB68355 AAS56615 AHY77896 AJP39292 AJU16491 |
REF | NP_012070 |
SP | P38886 |
TPG | DAA06893 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts