BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19368

Title: Structure of Pex14 in complex with Pex5 LVxEF motif   PubMed: 24235149

Deposition date: 2013-07-16 Original release date: 2013-11-26

Authors: Kooshapur, Hamed; Meyer, H.; Madl, T.; Sattler, M.

Citation: Neuhaus, Alexander; Kooshapur, Hamed; Wolf, Janina; Meyer, N. Helge; Madl, Tobias; Saidowsky, Jurgen; Hambruch, Eva; Lazam, Anissa; Jung, Martin; Sattler, Michael; Schliebs, Wolfgang; Erdmann, Ralf. "A Novel Pex14 Protein-interacting Site of Human Pex5 Is Critical for Matrix Protein Import into Peroxisomes."  J. Biol. Chem. 289, 437-448 (2014).

Assembly members:
PEROXISOMAL_MEMBRANE_PROTEIN_PEX14, polymer, 69 residues, 7445.4517 Da.
PEROXISOMAL_TARGETING_SIGNAL_1_RECEPTOR, polymer, 15 residues, 1701.7162 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
PEROXISOMAL_MEMBRANE_PROTEIN_PEX14: GAMATPGSENVLPREPLIAT AVKFLQNSRVRQSPLATRRA FLKKKGLTDEEIDMAFQQSG TAADEPSSL
PEROXISOMAL_TARGETING_SIGNAL_1_RECEPTOR: ASEDELVAEFLQDQN

Data sets:
Data typeCount
13C chemical shifts415
15N chemical shifts124
1H chemical shifts847

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PEROXISOMAL MEMBRANE PROTEIN PEX141
2PEROXISOMAL TARGETING SIGNAL 1 RECEPTOR2

Entities:

Entity 1, PEROXISOMAL MEMBRANE PROTEIN PEX14 69 residues - 7445.4517 Da.

1   GLYALAMETALATHRPROGLYSERGLUASN
2   VALLEUPROARGGLUPROLEUILEALATHR
3   ALAVALLYSPHELEUGLNASNSERARGVAL
4   ARGGLNSERPROLEUALATHRARGARGALA
5   PHELEULYSLYSLYSGLYLEUTHRASPGLU
6   GLUILEASPMETALAPHEGLNGLNSERGLY
7   THRALAALAASPGLUPROSERSERLEU

Entity 2, PEROXISOMAL TARGETING SIGNAL 1 RECEPTOR 15 residues - 1701.7162 Da.

1   ALASERGLUASPGLULEUVALALAGLUPHE
2   LEUGLNASPGLNASN

Samples:

sample_1: PEROXISOMAL MEMBRANE PROTEIN PEX14, [U-13C; U-15N], mM; PEROXISOMAL TARGETING SIGNAL 1 RECEPTOR, [U-13C; U-15N], mM

sample_conditions_1: ionic strength: 0.0 mM; pH: 6.500; pressure: 1 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
Experiment_1sample_1solutionsample_conditions_1

Software:

ARIA1.2_CNS vany, JP Linge, SI O Donoghue and M Nilges -

AutoDep v4.3 -

NMR spectrometers:

  • Bruker Avance 0 MHz

Related Database Links:

UNP PEX14_HUMAN PEX5_HUMAN
PDB
DBJ BAA36837 BAE00503 BAE88740 BAG35878 BAG51028
EMBL CAG29317 CAG46880
GB AAC39843 AAH06327 AAX36586 ABM83701 ABM86831
REF NP_001271931 NP_004556 XP_001118790 XP_001162201 XP_002811556 XP_004052675
SP O75381

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts