BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19369

Title: calbindin D9k(P47M+C80) Apo form   PubMed: 23933251

Deposition date: 2013-07-16 Original release date: 2014-02-13

Authors: Hembram, Dambarudhar; Haremaki, Takahiro; Hamatsu, Jumpei; Inoue, Jin; Kamoshida, Hajime; Ikeya, Teppei; Mishima, Masaki; Mikawa, Tsutomu; Hayashi, Nobuhiro; Shirakawa, Masahiro; Ito, Yutaka

Citation: Hembram, Dambarudhar Shiba Sankar; Haremaki, Takahiro; Hamatsu, Jumpei; Inoue, Jin; Kamoshida, Hajime; Ikeya, Teppei; Mishima, Masaki; Mikawa, Tsutomu; Hayashi, Nobuhiro; Shirakawa, Masahiro; Ito, Yutaka. "An in-cell NMR study of monitoring stress-induced increase of cytosolic Ca2+ concentration in HeLa cells."  Biochem. Biophys. Res. Commun. 438, 653-659 (2013).

Assembly members:
calbindin_D9k_Apo-form, polymer, 76 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
calbindin_D9k_Apo-form: KSPEELKRIFEKYAAKEGDP DQLSKDELKLLIQAEFPSLL KGMNTLDDLFQELDKNGDGE VSFEEFQVLVKKISQC

Data sets:
Data typeCount
13C chemical shifts219
15N chemical shifts71
1H chemical shifts71

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1calbindin D9k Apo-form1

Entities:

Entity 1, calbindin D9k Apo-form 76 residues - Formula weight is not available

1   LYSSERPROGLUGLULEULYSARGILEPHE
2   GLULYSTYRALAALALYSGLUGLYASPPRO
3   ASPGLNLEUSERLYSASPGLULEULYSLEU
4   LEUILEGLNALAGLUPHEPROSERLEULEU
5   LYSGLYMETASNTHRLEUASPASPLEUPHE
6   GLNGLULEUASPLYSASNGLYASPGLYGLU
7   VALSERPHEGLUGLUPHEGLNVALLEUVAL
8   LYSLYSILESERGLNCYS

Samples:

sample: calbindin D9k Apo-form, [U-100% 13C; U-100% 15N], 1.5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.15 M; pH: 7.4; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsampleisotropicsample_conditions_1
3D HNCOsampleisotropicsample_conditions_1
3D CBCA(CO)NHsampleisotropicsample_conditions_1
3D CBCANHsampleisotropicsample_conditions_1
3D HN(CA)COsampleisotropicsample_conditions_1

Software:

AZARA v2.8, Boucher - processing

ANALYSIS v2.2.2, CCPN - chemical shift assignment

QME, Daniel O, . - processing

TOPSPIN v3.1, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 19370 19371
DBJ BAI46392
EMBL CAA46699
GB AAA35637 AAA35638 AAI11918 AAI12175 EAW98916
REF NP_004048 XP_001102900 XP_001139782 XP_002831466 XP_003261140
SP P29377

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts