BMRB Entry 19396
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19396
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Title: Lasiocepsin PubMed: 24339323
Deposition date: 2013-07-30 Original release date: 2014-01-13
Authors: Monincova, Lenka; Budesinsky, Milos; Cujova, Sabina; Cerovsky, Vaclav; Veverka, Vaclav
Citation: Monincova, Lenka; Budinsky, Milos; Cujova, Sabina; Ceovsky, Vaclav; Veverka, Vaclav. "Structural basis for antimicrobial activity of lasiocepsin." Chembiochem 15, 301-308 (2014).
Assembly members:
lasiocepsin, polymer, 27 residues, 2906.848 Da.
Natural source: Common Name: bees Taxonomy ID: 88510 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Lasioglossum laticeps
Experimental source: Production method: chemical synthesis Host organism: na
Entity Sequences (FASTA):
lasiocepsin: GLPRKILCAIAKKKGKCKGP
LKLVCKC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 90 |
| 15N chemical shifts | 25 |
| 1H chemical shifts | 212 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Lasiocepsin | 1 |
Entities:
Entity 1, Lasiocepsin 27 residues - 2906.848 Da.
| 1 | GLY | LEU | PRO | ARG | LYS | ILE | LEU | CYS | ALA | ILE | ||||
| 2 | ALA | LYS | LYS | LYS | GLY | LYS | CYS | LYS | GLY | PRO | ||||
| 3 | LEU | LYS | LEU | VAL | CYS | LYS | CYS |
Samples:
sample_1: H2O 90%; D2O 10%; lasiocepsin 4 mM
sample_conditions_1: ionic strength: 0 M; pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection, processing
CYANA, Guntert, Mumenthaler and Wuthrich - refinement
SPARKY, Goddard - data analysis
AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| PDB |
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