BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19398

Title: Solution structure of the forkhead domain of Brugia malayi DAF-16a

Deposition date: 2013-07-30 Original release date: 2013-09-10

Authors: Veldkamp, C.; Peterson, F.; Casper, S.; Schoeller, S.

Citation: Veldkamp, C.; Peterson, F.; Casper, S.; Schoeller, S.. "Solution structure of the forkhead domain of Brugia malayi DAF-16a"  To be published ., .-..

Assembly members:
Bm-DAF-16a, polymer, 101 residues, 11673.083 Da.

Natural source:   Common Name: nematodes   Taxonomy ID: 6279   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Brugia malayi

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Bm-DAF-16a: NPWGAESYSDLIAKALKSTF DGRMRLNEIYNWFASNVPYF GNRTSQEQSAGWKNSIRHNL SLHSRFMRIQNEGAGKSSWW VINPDAKPGRNPRRQRSATL E

Data sets:
Data typeCount
13C chemical shifts423
15N chemical shifts109
1H chemical shifts687

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Forkhead DNA binding domain of Brugia malayi DAF-16a1

Entities:

Entity 1, Forkhead DNA binding domain of Brugia malayi DAF-16a 101 residues - 11673.083 Da.

1   ASNPROTRPGLYALAGLUSERTYRSERASP
2   LEUILEALALYSALALEULYSSERTHRPHE
3   ASPGLYARGMETARGLEUASNGLUILETYR
4   ASNTRPPHEALASERASNVALPROTYRPHE
5   GLYASNARGTHRSERGLNGLUGLNSERALA
6   GLYTRPLYSASNSERILEARGHISASNLEU
7   SERLEUHISSERARGPHEMETARGILEGLN
8   ASNGLUGLYALAGLYLYSSERSERTRPTRP
9   VALILEASNPROASPALALYSPROGLYARG
10   ASNPROARGARGGLNARGSERALATHRLEU
11   GLU

Samples:

sample: Forkhead box DNA binding domain of Brugia malayi DAF-16a, [U-98% 13C; U-98% 15N], 0.5 mM; H2O 90%; D20 10%; sodium phosphate 20 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 200 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D_15N-separated_NOESYsampleisotropicsample_conditions_1
3D_13C-separated_NOESYsampleisotropicsample_conditions_1
3D_13C-separated_NOESY (AROMATIC)sampleisotropicsample_conditions_1

Software:

X-PLOR NIH v2.9.3, SCHWIETERS,C.D.,KUSZEWSKI,J.J.,TJANDRA,N.,CLORE,G.M. - refinement

TOPSPIN v2.1, Bruker - collection

NMRPipe v2007, Delagio,F. et al. - processing

XEASY v1.3, Eccles, C., Guntert, P., Billeter, M., Wuthrich, K. - data analysis

GARANT v2.1, C. Bartels - data analysis

CARA v1.8.4, Keller, R. - data analysis

CYANA v3.0, Guntert, P. - structural calculation

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
EMBL CDQ02878
GB EJW78700
REF XP_001901487

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts