BMRB Entry 19477
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19477
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Title: Backbone 1H and 15N Chemical Shift Assignments for P130 Cas substrate domain PubMed: 24052412
Deposition date: 2013-09-05 Original release date: 2014-02-12
Authors: Liu, Xiao
Citation: Liu, Xiao; Yang, Daiwen. "HN(CA)N and HN(COCA)N experiments for assignment of large disordered proteins." J. Biomol. NMR 57, 83-89 (2013).
Assembly members:
P130Cas_substrate_domian, polymer, 306 residues, Formula weight is not available
Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
P130Cas_substrate_domian: PDNVYLVPTPSKTQQGLYQA
PGPNPQFQSPPAKQTSTFSK
QTPHHSFPSPATDLYQVPPG
PGSPAQDIYQVPPSAGTGHD
IYQVPPSLDTRSWEGTKPPA
KVVVPTRVGQGYVYEASQAE
QDEYDTPRHLLAPGSQDIYD
VPPVRGLLPNQYGQEVYDTP
PMAVKGPNGRDPLLDVYDVP
PSVEKGLPPSNHHSVYDVPP
SVSKDVPDGPLLREETYDVP
PAFAKPKPFDPTRHPLILAA
PPPDSPPAEDVYDVPPPAPD
LYDVPPGLRRPGPGTLYDVP
RERVLPPEVADGSVIDDGVY
AVPPPA
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 238 |
| 1H chemical shifts | 238 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | P130Cas SD, chain 1 | 1 |
| 2 | P130Cas SD, chain 2 | 1 |
Entities:
Entity 1, P130Cas SD, chain 1 306 residues - Formula weight is not available
| 1 | PRO | ASP | ASN | VAL | TYR | LEU | VAL | PRO | THR | PRO | ||||
| 2 | SER | LYS | THR | GLN | GLN | GLY | LEU | TYR | GLN | ALA | ||||
| 3 | PRO | GLY | PRO | ASN | PRO | GLN | PHE | GLN | SER | PRO | ||||
| 4 | PRO | ALA | LYS | GLN | THR | SER | THR | PHE | SER | LYS | ||||
| 5 | GLN | THR | PRO | HIS | HIS | SER | PHE | PRO | SER | PRO | ||||
| 6 | ALA | THR | ASP | LEU | TYR | GLN | VAL | PRO | PRO | GLY | ||||
| 7 | PRO | GLY | SER | PRO | ALA | GLN | ASP | ILE | TYR | GLN | ||||
| 8 | VAL | PRO | PRO | SER | ALA | GLY | THR | GLY | HIS | ASP | ||||
| 9 | ILE | TYR | GLN | VAL | PRO | PRO | SER | LEU | ASP | THR | ||||
| 10 | ARG | SER | TRP | GLU | GLY | THR | LYS | PRO | PRO | ALA | ||||
| 11 | LYS | VAL | VAL | VAL | PRO | THR | ARG | VAL | GLY | GLN | ||||
| 12 | GLY | TYR | VAL | TYR | GLU | ALA | SER | GLN | ALA | GLU | ||||
| 13 | GLN | ASP | GLU | TYR | ASP | THR | PRO | ARG | HIS | LEU | ||||
| 14 | LEU | ALA | PRO | GLY | SER | GLN | ASP | ILE | TYR | ASP | ||||
| 15 | VAL | PRO | PRO | VAL | ARG | GLY | LEU | LEU | PRO | ASN | ||||
| 16 | GLN | TYR | GLY | GLN | GLU | VAL | TYR | ASP | THR | PRO | ||||
| 17 | PRO | MET | ALA | VAL | LYS | GLY | PRO | ASN | GLY | ARG | ||||
| 18 | ASP | PRO | LEU | LEU | ASP | VAL | TYR | ASP | VAL | PRO | ||||
| 19 | PRO | SER | VAL | GLU | LYS | GLY | LEU | PRO | PRO | SER | ||||
| 20 | ASN | HIS | HIS | SER | VAL | TYR | ASP | VAL | PRO | PRO | ||||
| 21 | SER | VAL | SER | LYS | ASP | VAL | PRO | ASP | GLY | PRO | ||||
| 22 | LEU | LEU | ARG | GLU | GLU | THR | TYR | ASP | VAL | PRO | ||||
| 23 | PRO | ALA | PHE | ALA | LYS | PRO | LYS | PRO | PHE | ASP | ||||
| 24 | PRO | THR | ARG | HIS | PRO | LEU | ILE | LEU | ALA | ALA | ||||
| 25 | PRO | PRO | PRO | ASP | SER | PRO | PRO | ALA | GLU | ASP | ||||
| 26 | VAL | TYR | ASP | VAL | PRO | PRO | PRO | ALA | PRO | ASP | ||||
| 27 | LEU | TYR | ASP | VAL | PRO | PRO | GLY | LEU | ARG | ARG | ||||
| 28 | PRO | GLY | PRO | GLY | THR | LEU | TYR | ASP | VAL | PRO | ||||
| 29 | ARG | GLU | ARG | VAL | LEU | PRO | PRO | GLU | VAL | ALA | ||||
| 30 | ASP | GLY | SER | VAL | ILE | ASP | ASP | GLY | VAL | TYR | ||||
| 31 | ALA | VAL | PRO | PRO | PRO | ALA |
Samples:
P130CasSD_1: P130Cas substrate domian, [U-100% 13C; U-100% 15N], 1 mM
sample_conditions_1: ionic strength: 20 mM; pH: 6.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | P130CasSD_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)N | P130CasSD_1 | isotropic | sample_conditions_1 |
| 3D HN(COCA)N | P130CasSD_1 | isotropic | sample_conditions_1 |
Software:
NMRspy, Zheng, Liu and Yang - chemical shift assignment
NMR spectrometers:
- Bruker Avance 800 MHz
Related Database Links:
| DBJ | BAA06169 BAA06170 |
| GB | EDL92583 EDL92584 EDL92585 |
| REF | NP_037063 XP_006255690 XP_006255691 |
| SP | Q63767 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts