BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19556

Title: Solution structure of the C-terminally encoded peptide of the model plant host Medicago truncatula - CEP1   PubMed: 24211833

Deposition date: 2013-10-14 Original release date: 2013-12-09

Authors: Bobay, Benjamin; DiGennaro, Peter; McK. Bird, David

Citation: Bobay, Benjamin; DiGennaro, Peter; Scholl, Elizabeth; Imin, Nijat; Djordjevic, Michael; McK. Bird, David. "Solution NMR studies of the plant peptide hormone CEP inform function"  FEBS Lett. 587, 3979-3985 (2013).

Assembly members:
entity, polymer, 15 residues, 1499.576 Da.

Natural source:   Common Name: Barrel medic   Taxonomy ID: not available   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Medicago truncatula

Experimental source:   Production method: chemical synthesis   Host organism: synthetic

Entity Sequences (FASTA):
entity: AFQXTTPGNSXGVGH

Data sets:
Data typeCount
13C chemical shifts25
15N chemical shifts14
1H chemical shifts75

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CEP11

Entities:

Entity 1, CEP1 15 residues - 1499.576 Da.

1   ALAPHEGLNHYPTHRTHRPROGLYASNSER
2   HYPGLYVALGLYHIS

Samples:

sample_1: entity 4 mg/mL; DSS 10 uM; H2O 90%; D20 10%

sample_conditions_1: ionic strength: 0 M; pH: 7.0; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

ARIA, Linge, O, . - refinement, structure solution

NMR spectrometers:

  • Bruker Avance 700 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts