BMRB Entry 19569
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19569
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Title: NMR chemical shift assignments of mouse Vps28 C-terminal domain PubMed: 24366722
Deposition date: 2013-10-21 Original release date: 2014-02-12
Authors: Peterson, Tabitha; Fowler, Andrew; Piper, Robert; Yu, Liping
Citation: Peterson, Tabitha; Yu, Liping; Piper, Robert. "Backbone and side-chain NMR assignments for the C-terminal domain of mammalian Vps28." Biomol. NMR Assignments ., .-. (2013).
Assembly members:
Vps28C, polymer, 105 residues, Formula weight is not available
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Vps28C: GIDPFTMNLNRCIADVVSLF
ITVMDKLRLEIRAMDEIQPD
LRELMETMHRMSHLPPDFEG
RQTVSQWLQTLSGMSASDEL
DDSQVRQMLFDLESAYNAFN
RFLHA
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 433 |
15N chemical shifts | 105 |
1H chemical shifts | 712 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Vps28C | 1 |
Entities:
Entity 1, Vps28C 105 residues - Formula weight is not available
The N-terminal residues GIDPFTM are derived from affinity purification tag
1 | GLY | ILE | ASP | PRO | PHE | THR | MET | ASN | LEU | ASN | ||||
2 | ARG | CYS | ILE | ALA | ASP | VAL | VAL | SER | LEU | PHE | ||||
3 | ILE | THR | VAL | MET | ASP | LYS | LEU | ARG | LEU | GLU | ||||
4 | ILE | ARG | ALA | MET | ASP | GLU | ILE | GLN | PRO | ASP | ||||
5 | LEU | ARG | GLU | LEU | MET | GLU | THR | MET | HIS | ARG | ||||
6 | MET | SER | HIS | LEU | PRO | PRO | ASP | PHE | GLU | GLY | ||||
7 | ARG | GLN | THR | VAL | SER | GLN | TRP | LEU | GLN | THR | ||||
8 | LEU | SER | GLY | MET | SER | ALA | SER | ASP | GLU | LEU | ||||
9 | ASP | ASP | SER | GLN | VAL | ARG | GLN | MET | LEU | PHE | ||||
10 | ASP | LEU | GLU | SER | ALA | TYR | ASN | ALA | PHE | ASN | ||||
11 | ARG | PHE | LEU | HIS | ALA |
Samples:
sample_1: Vps28C, [U-98% 13C; U-98% 15N], 600 uM; sodium phosphate 40 mM; sodium chloride 50 mM; sodium azide 3 mM; DTT 5 mM
sample_2: Vps28C, [U-98% 13C; U-98% 15N], 600 uM; sodium phosphate 40 mM; sodium chloride 50 mM; sodium azide 3 mM; deuterated DTT 5 mM
sample_conditions_1: pH: 6.95; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HMQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HCCH-TOCSY | sample_2 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY | sample_2 | isotropic | sample_conditions_1 |
Software:
NMRView v08.01.00.00, Johnson, One Moon Scientific - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
TOPSPIN v2.1, Bruker Biospin - collection
NMR spectrometers:
- Bruker Avance II 500 MHz
- Bruker Avance II 800 MHz
Related Database Links:
EMBL | Q9D1C8 |
DBJ | BAB22945 BAC25042 |
GB | AAF00499 AAH06485 AAH13535 AAH19321 AAI02079 |
REF | NP_001030581 NP_001123964 NP_001253325 NP_001292597 NP_057292 |
SP | B5DEN9 Q3T178 Q9D1C8 Q9UK41 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts