BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules 		Member of WWPDB

BMRB Entry 19671

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19671

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Title: 3rC34Y, variant of Imunoglobulin light-chain of lambda 3   PubMed: 24816896

Deposition date: 2013-12-10 Original release date: 2015-04-15

Authors: Gil-Rodriguez, Paloma; Amero, Carlos

Citation: Gil-Rodriguez, Paloma; Amero, Carlos. "(1)H, (15)N and (13)C resonance assignments for 3rC and 3rCWP: amyloidogenic variants of imunoglobulin lambda 3 light-chain"  Biomol NMR Assign. 9, 139-142 (2015).

Assembly members:
3rC34Y, polymer, 107 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
3rC34Y: SYELTQPPSVSVSPGQTASI TCSGDKLGDKYAYWYQQKPG QSPVLVIYQDSKRPSGIPER FSGSNSGNTATLTISGTQAM DEADYYCQAWDSSTAVVFGG GTKLTVL

Data sets:
Data typeCount
13C chemical shifts273
15N chemical shifts91
1H chemical shifts458

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
13rC34Y1

Entities:

Entity 1, 3rC34Y 107 residues - Formula weight is not available

1   SERTYRGLULEUTHRGLNPROPROSERVAL
2   SERVALSERPROGLYGLNTHRALASERILE
3   THRCYSSERGLYASPLYSLEUGLYASPLYS
4   TYRALATYRTRPTYRGLNGLNLYSPROGLY
5   GLNSERPROVALLEUVALILETYRGLNASP
6   SERLYSARGPROSERGLYILEPROGLUARG
7   PHESERGLYSERASNSERGLYASNTHRALA
8   THRLEUTHRILESERGLYTHRGLNALAMET
9   ASPGLUALAASPTYRTYRCYSGLNALATRP
10   ASPSERSERTHRALAVALVALPHEGLYGLY
11   GLYTHRLYSLEUTHRVALLEU

Samples:

sample_1: sodium phosphate 20 mM; sodium chloride 20 mM; 3rC34Y, [U-15N], 1 ± 0.3 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_2: sodium phosphate 20 mM; sodium chloride 20 mM; 3rC34Y, [U-13C; U-15N], 1 ± 0.3 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_3: TRIS 20 mM; 3rC34Y, [U-13C; U-15N], 1 ± 0.3 mM; sodium chloride 20 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: ionic strength: 0.02 M; pH: 6; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 0.02 M; pH: 6; pressure: 1 atm; temperature: 303 K

sample_conditions_3: ionic strength: 0.02 M; pH: 8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_3isotropicsample_conditions_3
2D 1H-15N HSQCsample_1isotropicsample_conditions_2
3D CBCA(CO)NHsample_3isotropicsample_conditions_3
3D HNCOsample_3isotropicsample_conditions_3
3D HNCAsample_3isotropicsample_conditions_3
3D HNCACBsample_3isotropicsample_conditions_3
3D HNCACBsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D HBHA(CO)NHsample_2isotropicsample_conditions_2
3D HNCACBsample_2isotropicsample_conditions_2
3D HNCOsample_2isotropicsample_conditions_2
3D C(CO)NHsample_2isotropicsample_conditions_2
3D 1H-15N TOCSYsample_2isotropicsample_conditions_1

Software:

NMRPipe v7.9, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CARA v1.8.4, Keller and Wuthrich - data analysis

NMR spectrometers:

  • Varian INOVA 700 MHz

Related Database Links:

PDB
BMRB 19641
DBJ BAA20022 BAC01665 BAC01796
EMBL CAA12203 CAA80216 CAA85623 CAC10553 CAC10914
GB AAA19492 AAB00975 AAC16875 AAD01813 AAD01814
PIR S16440 S36065
PRF 2001180C

Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone or all simulated shifts