BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19674

Title: Solution structure of the CLAVATA encoded peptide of Arabidopsis thaliana - AtCLE10

Deposition date: 2013-12-16 Original release date: 2015-01-12

Authors: Bobay, Benjamin; DiGennaro, Peter; Bird, David

Citation: DiGennaro, Peter; Bobay, Benjamin; Mck Bird, David. "Inferring function of CLE peptides from high resolution tertiary structures"  Proc. Natl. Acad. Sci. U.S.A. ., .-..

Assembly members:
entity, polymer, 12 residues, 1302.495 Da.

Natural source:   Common Name: thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:   Production method: chemical synthesis   Host organism: Synthetic

Entity Sequences (FASTA):
entity: RLVPSGPNPLHN

Data sets:
Data typeCount
13C chemical shifts38
15N chemical shifts11
1H chemical shifts74

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CLAVATA encoded peptide of Arabidopsis thaliana - AtCLE101

Entities:

Entity 1, CLAVATA encoded peptide of Arabidopsis thaliana - AtCLE10 12 residues - 1302.495 Da.

1   ARGLEUVALPROSERGLYPROASNPROLEU
2   HISASN

Samples:

sample_1: entity 4 mg/mL; sodium chloride 137 mM; potassium chloride 2.7 mM; sodium phosphate 10 mM; potassium phosphate 1.8 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 140 mM; pH: 7.0; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

ARIA v2.3, Linge, O, . - refinement, structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 700 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts