BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19710

Title: Solution structure of CDYL2 chromodomain

Deposition date: 2013-12-28 Original release date: 2014-04-22

Authors: Qin, Su; Houliston, Scott; Arrowsmith, Cheryl; Edwards, Aled; Wu, Hong; Min, Jinrong

Citation: Qin, Su; Houliston, Scott; Arrowsmith, Cheryl; Wu, Hong; Min, Jinrong. "Solution structure of CDYL2 chromodomain"  Not known ., .-..

Assembly members:
entity, polymer, 64 residues, 7695.667 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: GASGDLYEVERIVDKRKNKK GKWEYLIRWKGYGSTEDTWE PEHHLLHCEEFIDEFNGLHM SKDK

Data sets:
Data typeCount
13C chemical shifts232
15N chemical shifts54
1H chemical shifts366

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CDYL2 chromodomain1

Entities:

Entity 1, CDYL2 chromodomain 64 residues - 7695.667 Da.

1   GLYALASERGLYASPLEUTYRGLUVALGLU
2   ARGILEVALASPLYSARGLYSASNLYSLYS
3   GLYLYSTRPGLUTYRLEUILEARGTRPLYS
4   GLYTYRGLYSERTHRGLUASPTHRTRPGLU
5   PROGLUHISHISLEULEUHISCYSGLUGLU
6   PHEILEASPGLUPHEASNGLYLEUHISMET
7   SERLYSASPLYS

Samples:

sample_1: TRIS 20 mM; sodium chloride 150 mM; DTT 2 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: ionic strength: 0.15 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, data analysis

TALOS, Cornilescu, Delaglio and Bax - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

fmcgui v2.5, Lemak A. - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAB29852 BAC04720 BAE24934 BAJ21089
GB AAH58956 AAH69440 AAI00804 AAI00805 AAI00806
REF NP_083717 NP_689555 XP_003791470 XP_004392121 XP_004690411
SP Q8N8U2 Q9D5D8

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts