BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19810

Title: Solution structure of BmKTX-D19K

Deposition date: 2014-02-21 Original release date: 2015-02-23

Authors: Hong, Jing; Lin, Donghai; Chen, Zongyun; Wu, Yingliang

Citation: Hong, Jing; Lin, Donghai; Chen, Zongyun; Wu, Yingliang. "Solution structure of BmKTX-D19K"  Not known ., .-..

Assembly members:
entity, polymer, 37 residues, 3991.932 Da.

Natural source:   Common Name: scorpion   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: VGINVKCKHSGQCLKPCKKA GMRFGKCINGKCDCTPK

Data sets:
Data typeCount
1H chemical shifts246

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BmKTX-D19K1

Entities:

Entity 1, BmKTX-D19K 37 residues - 3991.932 Da.

1   VALGLYILEASNVALLYSCYSLYSHISSER
2   GLYGLNCYSLEULYSPROCYSLYSLYSALA
3   GLYMETARGPHEGLYLYSCYSILEASNGLY
4   LYSCYSASPCYSTHRPROLYS

Samples:

sample_1: entity 2 mM; D2O 10%; sodium phosphate 25 mM; H2O 90%

sample_conditions_1: ionic strength: 0.025 M; pH: 4.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1

Software:

ARIA, Linge, O, . - chemical shift assignment

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

SPARKY, Goddard - peak picking

ProcheckNMR, Laskowski and MacArthur - quality check

NMR spectrometers:

  • Bruker AMX 600 MHz

Related Database Links:

BMRB 19813
PDB
GB AAD47376 AAF63970
SP C0HJQ6 Q9NII7