BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19913

Title: NMR Structure of KDM5B PHD1 finger   PubMed: 24952722

Deposition date: 2014-04-16 Original release date: 2014-08-04

Authors: Zhang, Y.; Yang, H.; Guo, X.; Rong, N.; Song, Y.; Xu, Y.; Lan, W.; Xu, Y.; Cao, C.

Citation: Zhang, Y.; Yang, H.; Guo, X.; Rong, N.; Song, Y.; Xu, Y.; Lan, W.; Xu, Y.; Cao, C.. "The PHD1 finger of KDM5B recognizes unmodified H3K4 during the demethylation of histone H3K4me2/3 by KDM5B"  Protein Cell ., .-. (2014).

Assembly members:
PHD1_finger_of_KDM5B, polymer, 55 residues, 6133.026 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
PHD1_finger_of_KDM5B: AVDLYVCLLCGSGNDEDRLL LCDGCDDSYHTFCLIPPLHD VPKGDWRCPKCLAQE

Data sets:
Data typeCount
13C chemical shifts189
15N chemical shifts51
1H chemical shifts301

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PHD1 finger of KDM5B1
2ZINC ION_12
3ZINC ION_22

Entities:

Entity 1, PHD1 finger of KDM5B 55 residues - 6133.026 Da.

1   ALAVALASPLEUTYRVALCYSLEULEUCYS
2   GLYSERGLYASNASPGLUASPARGLEULEU
3   LEUCYSASPGLYCYSASPASPSERTYRHIS
4   THRPHECYSLEUILEPROPROLEUHISASP
5   VALPROLYSGLYASPTRPARGCYSPROLYS
6   CYSLEUALAGLNGLU

Entity 2, ZINC ION_1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: PHD1 finger of KDM5B, [U-13C; U-15N], 0.8 mM; Phosphate buffer 50 mM; D2O 10%; H2O 90%

sample_2: PHD1 finger of KDM5B, [U-13C; U-15N], 0.8 mM; Phosphate buffer 50 mM; D2O 100%

sample_conditions_1: ionic strength: 100 mM; pH: 7.2; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D_15N-separated_NOESYsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_2isotropicsample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

NMR spectrometers:

  • Agilent DD2 600 MHz

Related Database Links:

BMRB 19914
PDB
DBJ BAC30898 BAD90482 BAE37363 BAE89761 BAG53706
EMBL CAB43532 CAB61368 CAB63108 CAH65222
GB AAD16061 AAH48180 AAH57318 AAI56050 AAI57032
REF NP_001026200 NP_001100647 NP_001300971 NP_006609 NP_690855
SP Q5F3R2 Q80Y84 Q9UGL1
TPG DAA21261

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts