BMRB Entry 19951
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19951
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Title: Resonance assignments and secondary structure of apolipoprotein E C-terminal domain in complex with DHPC
Deposition date: 2014-05-02 Original release date: 2015-05-05
Authors: Lo, Chi-Jen; Chen, Yi-Chen; Chang, Chi-Fon; Shiao, Ming-Shi; Huang, Hsien-bin; Lin, Ta-Hsien
Citation: Lo, Chi-Jen; Chyan, Chia-Lin; Chen, Yi-Chen; Chang, Chi-Fon; Huang, Hsien-bin; Lin, Ta-Hsien. "Resonance assignments and secondary structure of apolipoprotein E C-terminal domain in DHPC micelles" Biomol. NMR Assign. 9, 187-190 (2015).
Assembly members:
apoE(195-299), polymer, 108 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
apoE(195-299): GSMVGSLAGQPLQERAQAWG
ERLRARMEEMGSRTRDRLDE
VKEQVAEVRAKLEEQAQQIR
LQAEAFQARLKSWFEPLVED
MQRQWAGLVEKVQAAVGTSA
APVPSDNH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 306 |
| 15N chemical shifts | 94 |
| 1H chemical shifts | 212 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | apoE(195-299) | 1 |
Entities:
Entity 1, apoE(195-299) 108 residues - Formula weight is not available
| 1 | GLY | SER | MET | VAL | GLY | SER | LEU | ALA | GLY | GLN | ||||
| 2 | PRO | LEU | GLN | GLU | ARG | ALA | GLN | ALA | TRP | GLY | ||||
| 3 | GLU | ARG | LEU | ARG | ALA | ARG | MET | GLU | GLU | MET | ||||
| 4 | GLY | SER | ARG | THR | ARG | ASP | ARG | LEU | ASP | GLU | ||||
| 5 | VAL | LYS | GLU | GLN | VAL | ALA | GLU | VAL | ARG | ALA | ||||
| 6 | LYS | LEU | GLU | GLU | GLN | ALA | GLN | GLN | ILE | ARG | ||||
| 7 | LEU | GLN | ALA | GLU | ALA | PHE | GLN | ALA | ARG | LEU | ||||
| 8 | LYS | SER | TRP | PHE | GLU | PRO | LEU | VAL | GLU | ASP | ||||
| 9 | MET | GLN | ARG | GLN | TRP | ALA | GLY | LEU | VAL | GLU | ||||
| 10 | LYS | VAL | GLN | ALA | ALA | VAL | GLY | THR | SER | ALA | ||||
| 11 | ALA | PRO | VAL | PRO | SER | ASP | ASN | HIS |
Samples:
sample_1: apoE(195-299), [U-13C; U-15N; U-2H], 1 mM; potassium phosphate 50 mM; sodium azide 0.02 mM; TSP, [U-2H], 0.02 mM; EDTA 0.5 mM; dihexanoyl phosphatidylcholine (DHPC) micelles mM
sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CBCA)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
AURELIA v3.6, Neidig, Geyer, Gorler, Antz, Saffrich, Beneicke, Kalbitzer - chemical shift assignment
TOPSPIN v3.1, Bruker Biospin - processing
NMR spectrometers:
- Bruker Avance 800 MHz
Related Database Links:
| DBJ | BAG37412 BAG72927 |
| GB | AAB59397 AAB59518 AAB59546 AAD02505 AAG27089 |
| PRF | 1506383A |
| REF | NP_000032 NP_001289617 NP_001289618 NP_001289619 NP_001289620 |
| SP | P02649 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts