BMRB Entry 20034
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20034
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Title: Solution NMR Structure of 4F Associated With DMPC Disc PubMed: 18845546
Deposition date: 2008-08-14 Original release date: 2008-10-14
Authors: Mishra, Vinod
Citation: Mishra, Vinod; Palgunachari, Mayakonda; Krishna, N.; Glushka, John; Segrest, Jere; Anantharamaiah, G.. "Effect of leucine to phenylalanine substitution on the nonpolar face of a class a amphipathic helical peptide on its interaction with lipid: High-resolution solution nmr studies of 4F:Dimyristoylphosphatidylcholine discoidal complex" J. Biol. Chem. 283, 34393-34402 (2008).
Assembly members:
4F, polymer, 19 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
4F: XDWFKAFYDKVAEKFKEAF
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 137 |
conformer coordinate sets | 1 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | 4F Associated With DMPC Disc | 1 |
Entities:
Entity 1, 4F Associated With DMPC Disc 19 residues - Formula weight is not available
1 | ACE | ASP | TRP | PHE | LYS | ALA | PHE | TYR | ASP | LYS | ||||
2 | VAL | ALA | GLU | LYS | PHE | LYS | GLU | ALA | PHE |
Samples:
sample_1: sodium phosphate 5 mM
sample_conditions_1: pH: 5.5; temperature: 310 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_1 |
2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR, Brunger - geometry optimization
NMR spectrometers:
- Bruker Avance 600 MHz
- Varian INOVA 900 MHz