BMRB Entry 20045
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20045
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: SOLUTION NMR STRUCTURE OF HUMAN IAPP-1-19 PubMed: 18989932
Deposition date: 2008-09-03 Original release date: 2009-04-04
Authors: NANGA, RAVI PRAKASH REDDY; BRENDER, JEFFREY; XU, JIADI; VEGLIA, GIANLUIGI; RAMAMOORTHY, AYYALUSAMY
Citation: NANGA, RAVI PRAKASH REDDY; BRENDER, JEFFREY; XU, JIADI; VEGLIA, GIANLUIGI; RAMAMOORTHY, AYYALUSAMY. "Structures of rat and human islet amyloid polypeptide IAPP(1-19) in micelles by NMR spectroscopy" Biochemistry 47, 12689-12697 (2008).
Assembly members:
IAPP, polymer, 19 residues, 2081.410 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
IAPP: KCNTATCATQRLANFLVHS
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 104 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | IAPP | 1 |
Entities:
Entity 1, IAPP 19 residues - 2081.410 Da.
1 | LYS | CYS | ASN | THR | ALA | THR | CYS | ALA | THR | GLN | ||||
2 | ARG | LEU | ALA | ASN | PHE | LEU | VAL | HIS | SER |
Samples:
sample_1: entity 1.625 mM; DPC, [U-100% 2H], 200 mM; sodium azide 0.02%; sodium phosphate 20 mM; sodium chloride 120 mM; D2O 10%; H2O 90%
sample_conditions_1: pH: 7.3; temperature: 303 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v2.1, Bruker Biospin - collection, processing
SPARKY, Goddard - chemical shift assignment, chemical shift calculation, data analysis
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution
TALOS, Cornilescu, Delaglio and Bax - refinement
ProcheckNMR, Laskowski and MacArthur - data analysis
NMR spectrometers:
- Bruker Avance 900 MHz