BMRB Entry 20080
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20080
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Title: Structure and interactions in membranes of human and bovine Catestatin by solution and solid-state NMR spectroscopy PubMed: 20103720
Deposition date: 2009-04-09 Original release date: 2010-03-01
Authors: Sugewara, Masae; Resende, Jarbas; Moraes, Cleria; Chich, Jean-Francois; Marquette, Arnaud; Metz-Boutigue, Marie-Helene; Bechinger, Burkhard
Citation: Sugawara, Masae; Resende, Jarbas; Moraes, Cleria Mendonca; Marquette, Arnaud; Chich, Jean-Francois; Metz-Boutigue, Marie-Helene; Bechinger, Burkhard. "Membrane structure and interactions of human catestatin by multidimensional solution and solid-state NMR spectroscopy." FASEB J. 24, 1737-1746 (2010).
Assembly members:
hCGA(G364S), polymer, 21 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
hCGA(G364S): SSMKLSFRARAYSFRGPGPQ
L
- assigned_chemical_shifts
- conformer_family_coord_set
Data type | Count |
1H chemical shifts | 132 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | hCGA(G364S) | 1 |
Entities:
Entity 1, hCGA(G364S) 21 residues - Formula weight is not available
1 | SER | SER | MET | LYS | LEU | SER | PHE | ARG | ALA | ARG | ||||
2 | ALA | TYR | SER | PHE | ARG | GLY | PRO | GLY | PRO | GLN | ||||
3 | LEU |
Samples:
sample_1: hCGA(G364S) 2.0 ± 0.1 mM; DPC-d38, [U-2H], 400 ± 1.0 mM; D2O, [U-2H], 95%; H2O 5.0%; PB 10 mM
sample_conditions_1: pH: 5.5; temperature: 298.0 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
xwinnmr, Bruker Biospin - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution
Molmol, Koradi, Billeter and Wuthrich - data analysis
NMR spectrometers:
- Bruker Avance 500 MHz