BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25162

Title: Carnolysin A2'   PubMed: 25207953

Deposition date: 2014-08-18 Original release date: 2014-09-25

Authors: Lohans, Christopher; Li, Jessica; Vederas, John

Citation: Lohans, Christopher; Li, Jessica; Vederas, John. "Structure and Biosynthesis of Carnolysin, a Homolog of Enterococcal Cytolysin with D-Amino Acids"  J. Am. Chem. Soc. 136, 13150-13153 (2014).

Assembly members:
CrnA2', polymer, 31 residues, Formula weight is not available

Natural source:   Common Name: firmicutes   Taxonomy ID: 2751   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Carnobacterium maltaromaticum

Experimental source:   Production method: purified from the natural source   Host organism: Carnobacterium maltaromaticum

Entity Sequences (FASTA):
CrnA2': GDVMPEAXPICAGFAXLMXX IGLVKXIKGKC

Data sets:
Data typeCount
1H chemical shifts166

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CrnA2'1

Entities:

Entity 1, CrnA2' 31 residues - Formula weight is not available

1   GLYASPVALMETPROGLUALADBUPROILE
2   CYSALAGLYPHEALADBBLEUMETDALDHA
3   ILEGLYLEUVALLYSDBBILELYSGLYLYS
4   CYS

Samples:

sample_1: CrnA2' 0.8 mM; DSS 0.01%; H2O 61%; D2O 6%; CD3OD 33%

sample_conditions_1: temperature: 273 K; pH: 6

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

NMR spectrometers:

  • Varian VXRS 700 MHz