BMRB Entry 25222

Name: Monomeric phospholamban, R14del mutant
Published: 2014-09-29

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25222

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Monomeric phospholamban, R14del mutant PubMed: 25251363

Deposition date: 2014-09-13 Original release date: 2014-09-29

Authors: Vostrikov, Vitaly; Soller, Kailey; Ha, Kim; Gopinath, Tata; Veglia, Gianluigi

Citation: Vostrikov, Vitaly; Soller, Kailey; Ha, Kim; Gopinath, T; Veglia, Gianluigi. "Effects of naturally occurring arginine 14 deletion on phospholamban conformational dynamics and membrane interactions." Biochim. Biophys. Acta 1848, 315-322 (2015).

Assembly members:
Phospholamban, polymer, 52 residues, Formula weight is not available

Natural source: Common Name: rabbit Taxonomy ID: 9986 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Oryctolagus cuniculus

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
Phospholamban: AMEKVQYLTRSAIRASTIEM PQQARQNLQNLFINFALILI FLLLIAIIVMLL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	49
1H chemical shifts	49

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Phospholamban	1

Entities:

Entity 1, Phospholamban 52 residues - Formula weight is not available

1

ALA

MET

GLU

LYS

VAL

GLN

TYR

LEU

THR

ARG

2

SER

ALA

ILE

ARG

ALA

SER

THR

ILE

GLU

MET

3

PRO

GLN

ALA

ARG

GLN

ASN

LEU

GLN

ASN

4

LEU

PHE

ILE

ASN

PHE

ALA

LEU

ILE

LEU

ILE

5

PHE

LEU

ILE

ALA

ILE

VAL

MET

6

LEU

Samples:

sample_1: Phospholamban, [U-100% 15N], 0.1 mM; DPC, [U-2H], 300 mM; sodium phosphate 20 mM; sodium chloride 120 mM; beta-mercaptoethanol 10 mM; sodium azide 0.02%; H2O 95%; D2O 5%

sample_conditions_1: pH: 6; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe v7.4, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMRJ, Varian - collection

SPARKY v3.114, Goddard - data analysis

NMR spectrometers:

Varian INOVA 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts