BMRB Entry 25292
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25292
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Title: HIV-1 reverse transcriptase N terminal 216 residues (Fingers and Palm subdomain) PubMed: 24574528
Deposition date: 2014-10-21 Original release date: 2014-11-21
Authors: Zheng, Xunhai; Mueller, Geoffry; London, Robert
Citation: Zheng, Xunhai; Pedersen, Lars; Gabel, Scott; Mueller, Geoffrey; Cuneo, Matthew; DeRose, Eugene; Krahn, Juno; London, Robert. "Selective unfolding of one Ribonuclease H domain of HIV reverse transcriptase is linked to homodimer formation" Nucleic Acids Res. 42, 5361-5377 (2014).
Assembly members:
RT216, polymer, 217 residues, Formula weight is not available
Natural source: Common Name: HIV Taxonomy ID: 11676 Superkingdom: Viruses Kingdom: not available Genus/species: Lentivirus not available
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
RT216: MPISPIETVPVKLKPGMDGP
KVKQWPLTEEKIKALVEICT
EMEKEGKISKIGPENPYNTP
VFAIKKKDSTKWRKLVDFRE
LNKRTQDFWEVQLGIPHPAG
LKKKKSVTVLDVGDAYFSVP
LDEDFRKYTAFTIPSINNET
PGIRYQYNVLPQGWKGSPAI
FQSSMTKILEPFRKQNPDIV
IYQYMDDLYVGSDLEIGQHR
TKIEELRQHLLRWGLTT
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 284 |
15N chemical shifts | 131 |
1H chemical shifts | 131 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | RT216 | 1 |
Entities:
Entity 1, RT216 217 residues - Formula weight is not available
1 | MET | PRO | ILE | SER | PRO | ILE | GLU | THR | VAL | PRO | ||||
2 | VAL | LYS | LEU | LYS | PRO | GLY | MET | ASP | GLY | PRO | ||||
3 | LYS | VAL | LYS | GLN | TRP | PRO | LEU | THR | GLU | GLU | ||||
4 | LYS | ILE | LYS | ALA | LEU | VAL | GLU | ILE | CYS | THR | ||||
5 | GLU | MET | GLU | LYS | GLU | GLY | LYS | ILE | SER | LYS | ||||
6 | ILE | GLY | PRO | GLU | ASN | PRO | TYR | ASN | THR | PRO | ||||
7 | VAL | PHE | ALA | ILE | LYS | LYS | LYS | ASP | SER | THR | ||||
8 | LYS | TRP | ARG | LYS | LEU | VAL | ASP | PHE | ARG | GLU | ||||
9 | LEU | ASN | LYS | ARG | THR | GLN | ASP | PHE | TRP | GLU | ||||
10 | VAL | GLN | LEU | GLY | ILE | PRO | HIS | PRO | ALA | GLY | ||||
11 | LEU | LYS | LYS | LYS | LYS | SER | VAL | THR | VAL | LEU | ||||
12 | ASP | VAL | GLY | ASP | ALA | TYR | PHE | SER | VAL | PRO | ||||
13 | LEU | ASP | GLU | ASP | PHE | ARG | LYS | TYR | THR | ALA | ||||
14 | PHE | THR | ILE | PRO | SER | ILE | ASN | ASN | GLU | THR | ||||
15 | PRO | GLY | ILE | ARG | TYR | GLN | TYR | ASN | VAL | LEU | ||||
16 | PRO | GLN | GLY | TRP | LYS | GLY | SER | PRO | ALA | ILE | ||||
17 | PHE | GLN | SER | SER | MET | THR | LYS | ILE | LEU | GLU | ||||
18 | PRO | PHE | ARG | LYS | GLN | ASN | PRO | ASP | ILE | VAL | ||||
19 | ILE | TYR | GLN | TYR | MET | ASP | ASP | LEU | TYR | VAL | ||||
20 | GLY | SER | ASP | LEU | GLU | ILE | GLY | GLN | HIS | ARG | ||||
21 | THR | LYS | ILE | GLU | GLU | LEU | ARG | GLN | HIS | LEU | ||||
22 | LEU | ARG | TRP | GLY | LEU | THR | THR |
Samples:
sample_1: RT216, [U-13C; U-15N; U-2H] ILE methyl protonated, .6 mM; TRIS, d-11, 50 mM; EDTA, d-16, 1 mM; DSS 0.2 mM; sodium azide 2%; H2O 90%; D2O 8%
sample_conditions_1: ionic strength: 1 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CB | sample_1 | isotropic | sample_conditions_1 |
3D HN(COCA)CB | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D CCCCOSY_CA_NNH | sample_1 | isotropic | sample_conditions_1 |
3D HCCCCOSY_CA_NNH | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - data analysis
VNMRJ, Varian - collection
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
PDB | |
DBJ | BAA00993 BAA12989 BAA12997 BAA24269 BAA24270 |
EMBL | CAA05982 CAA05984 CAA06947 CAA12651 CAA12656 |
GB | AAA44198 AAA44619 AAA44653 AAA44988 AAA45006 |
PIR | GNVWLV |
PRF | 1102247C 1103299E 1817237A |
REF | NP_057849 NP_705927 NP_789739 NP_789740 YP_001856242 |
SP | P03366 P03367 P03369 P04585 P04587 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts