BMRB Entry 25401
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25401
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Title: 1H, 15N 13C Chemical shift assignments for CCL28
Deposition date: 2014-12-19 Original release date: 2015-01-20
Authors: Thomas, Monica; Peterson, Francis; Volkman, Brian
Citation: Thomas, Monica; Buelow, Becky; Nevins, Amanda; Jones, Stephanie; Peterson, Francis; Gundry, Rebekah; Grayson, Mitchell; Volkman, Brian. "Structure-function analysis of CCL28 in post viral asthma" J. Biol. Chem. ., .-..
Assembly members:
CCL28, polymer, 108 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
CCL28: SEAILPIASSCCTEVSHHIS
RRLLERVNMCRIQRADGDCD
LAAVILHVKRRRICVSPHNH
TVKQWMKVQAAKKNGKGNVC
HRKKHHGKRNSNRAHQGKHE
TYGHKTPY
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 434 |
| 1H chemical shifts | 698 |
| 15N chemical shifts | 110 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CCL28 | 1 |
Entities:
Entity 1, CCL28 108 residues - Formula weight is not available
| 1 | SER | GLU | ALA | ILE | LEU | PRO | ILE | ALA | SER | SER | ||||
| 2 | CYS | CYS | THR | GLU | VAL | SER | HIS | HIS | ILE | SER | ||||
| 3 | ARG | ARG | LEU | LEU | GLU | ARG | VAL | ASN | MET | CYS | ||||
| 4 | ARG | ILE | GLN | ARG | ALA | ASP | GLY | ASP | CYS | ASP | ||||
| 5 | LEU | ALA | ALA | VAL | ILE | LEU | HIS | VAL | LYS | ARG | ||||
| 6 | ARG | ARG | ILE | CYS | VAL | SER | PRO | HIS | ASN | HIS | ||||
| 7 | THR | VAL | LYS | GLN | TRP | MET | LYS | VAL | GLN | ALA | ||||
| 8 | ALA | LYS | LYS | ASN | GLY | LYS | GLY | ASN | VAL | CYS | ||||
| 9 | HIS | ARG | LYS | LYS | HIS | HIS | GLY | LYS | ARG | ASN | ||||
| 10 | SER | ASN | ARG | ALA | HIS | GLN | GLY | LYS | HIS | GLU | ||||
| 11 | THR | TYR | GLY | HIS | LYS | THR | PRO | TYR |
Samples:
sample_1: CCL28, [U-99% 13C; U-99% 15N], 1 mM; sodium phosphate 20 mM; sodium azide 0.02%; D2O, [U-99% 2H], 10%
sample_conditions_1: temperature: 298 K; pH: 6.2; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - processing
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
XEASY, Bartels et al. - data analysis, chemical shift assignment
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| DBJ | BAG35056 BAJ20648 |
| GB | AAF87205 AAG16691 AAG43193 AAH62668 AAH69532 |
| REF | NP_001288802 NP_001288803 NP_001288804 NP_683513 XP_001135733 |
| SP | Q9NRJ3 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
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