BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25510

Title: NMR Solution structure of AIM2 PYD from Mus musculus   PubMed: 25888795

Deposition date: 2015-03-01 Original release date: 2015-04-21

Authors: Hou, Xianhui; Niu, Xiaogang

Citation: Hou, Xianhui; Niu, Xiaogang. "The NMR solution structure of AIM2 PYD domain from Mus musculus reveals a distinct 2-3 helix conformation from its human homologues"  Biochem. Biophys. Res. Commun. 461, 396-400 (2015).

Assembly members:
entity, polymer, 95 residues, 10963.646 Da.

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: MESEYREMLLLTGLDHITEE ELKRFKYFALTEFQIARSTL DVADRTELADHLIQSAGAAS AVTKAINIFQKLNYMHIANA LEEKKKEAERKLMTN

Data sets:
Data typeCount
13C chemical shifts378
15N chemical shifts94
1H chemical shifts589

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1AIM2 PYD1

Entities:

Entity 1, AIM2 PYD 95 residues - 10963.646 Da.

1   METGLUSERGLUTYRARGGLUMETLEULEU
2   LEUTHRGLYLEUASPHISILETHRGLUGLU
3   GLULEULYSARGPHELYSTYRPHEALALEU
4   THRGLUPHEGLNILEALAARGSERTHRLEU
5   ASPVALALAASPARGTHRGLULEUALAASP
6   HISLEUILEGLNSERALAGLYALAALASER
7   ALAVALTHRLYSALAILEASNILEPHEGLN
8   LYSLEUASNTYRMETHISILEALAASNALA
9   LEUGLUGLULYSLYSLYSGLUALAGLUARG
10   LYSLEUMETTHRASN

Samples:

sample_1: entity, [U-100% 13C; U-100% 15N], 0.5 mM; NaAc/HAc 20 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.02 M; pH: 4.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

  • Bruker Avance 700 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts