BMRB Entry 25676
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25676
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Backbone resonance assignments of the mutant NS4A N-terminal domain PubMed: 26197333
Deposition date: 2015-06-30 Original release date: 2015-07-24
Authors: Hung, Yu-Fu; Schwarten, Melanie; Hoffmann, Silke; Willbold, Dieter; Sklan, Ella; Koenig, Bernd
Citation: Hung, Yu-Fu; Schwarten, Melanie; Hoffmann, Silke; Willbold, Dieter; Sklan, Ella; Koenig, Bernd. "Amino Terminal Region of Dengue Virus NS4A Cytosolic Domain Binds to Highly Curved Liposomes." Viruses 7, 4119-4130 (2015).
Assembly members:
molecule_1, polymer, 48 residues, Formula weight is not available
Natural source: Common Name: Aeropyrum pernix Taxonomy ID: 56636 Superkingdom: Archaea Kingdom: not available Genus/species: Aeropyrum pernix
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
molecule_1: SLTLNEITEEGRLPTFMTQK
ARDALDNLAVLHTAEAGGRA
YNHALSEL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 134 |
| 15N chemical shifts | 41 |
| 1H chemical shifts | 41 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | NS4A mut | 1 |
Entities:
Entity 1, NS4A mut 48 residues - Formula weight is not available
| 1 | SER | LEU | THR | LEU | ASN | GLU | ILE | THR | GLU | GLU | ||||
| 2 | GLY | ARG | LEU | PRO | THR | PHE | MET | THR | GLN | LYS | ||||
| 3 | ALA | ARG | ASP | ALA | LEU | ASP | ASN | LEU | ALA | VAL | ||||
| 4 | LEU | HIS | THR | ALA | GLU | ALA | GLY | GLY | ARG | ALA | ||||
| 5 | TYR | ASN | HIS | ALA | LEU | SER | GLU | LEU |
Samples:
NMR_sample: mutNS4A, [U-13C; U-15N], 200 uM; Sodium Phosphate 50 mM; Sodium Azide 0.03%; Sodium Chloride 150 mM; H2O 90%; D2O, [U-2H], 10%
sample_conditions_1: ionic strength: 200 mM; pH: 6.8; pressure: 1.0 atm; temperature: 303.0 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | NMR_sample | isotropic | sample_conditions_1 |
| 3D HNCA | NMR_sample | isotropic | sample_conditions_1 |
| 3D HNCACO | NMR_sample | isotropic | sample_conditions_1 |
| 3D HNCACB | NMR_sample | isotropic | sample_conditions_1 |
| 3D HNCO | NMR_sample | isotropic | sample_conditions_1 |
Software:
CcpNmr_Analysis v2.4, CCPN - chemical shift assignment
NMR spectrometers:
- Bruker Avance III HD 700 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts