BMRB Entry 26600
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26600
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Title: Backbone 1H, 13C, 15N chemical shift assignments for T cell receptor N15 beta subunit PubMed: 26275917
Deposition date: 2015-07-06 Original release date: 2015-07-14
Authors: Mallis, Robert; Arthanari, Haribabu; Reinherz, Ellis; Wagner, Gerhard
Citation: Mallis, Robert; Reinherz, Ellis; Wagner, Gerhard; Arthanari, Haribabu. "Backbone Resonance Assignment of N15, N30 and D10 T cell Receptor beta subunits" Biomol. NMR Assign. 10, 35-39 (2016).
Assembly members:
N15_beta_TCR, polymer, 239 residues, Formula weight is not available
Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
N15_beta_TCR: DSGVVQSPRHIIKEKGGRSV
LTCIPISGHSNVVWYQQTLG
KELKFLIQHYEKVERDKGFL
PSRFSVQQFDDYHSEMNMSA
LELEDSAMYFCASSLRWGDE
QYFGPGTRLTVLEDLRNVTP
PKVSLFEPSKAEIANKQKAT
LVCLARGFFPDHVELSWWVN
GKEVHSGVSTDPQAYKESNY
SYSLSSRLRVSATFWHNPRN
HFRCQVQFHGLSEEDKWPEG
SPKPVTQNISAEAWGRADS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 580 |
| 15N chemical shifts | 198 |
| 1H chemical shifts | 198 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | beta subunit | 1 |
Entities:
Entity 1, beta subunit 239 residues - Formula weight is not available
| 1 | ASP | SER | GLY | VAL | VAL | GLN | SER | PRO | ARG | HIS | ||||
| 2 | ILE | ILE | LYS | GLU | LYS | GLY | GLY | ARG | SER | VAL | ||||
| 3 | LEU | THR | CYS | ILE | PRO | ILE | SER | GLY | HIS | SER | ||||
| 4 | ASN | VAL | VAL | TRP | TYR | GLN | GLN | THR | LEU | GLY | ||||
| 5 | LYS | GLU | LEU | LYS | PHE | LEU | ILE | GLN | HIS | TYR | ||||
| 6 | GLU | LYS | VAL | GLU | ARG | ASP | LYS | GLY | PHE | LEU | ||||
| 7 | PRO | SER | ARG | PHE | SER | VAL | GLN | GLN | PHE | ASP | ||||
| 8 | ASP | TYR | HIS | SER | GLU | MET | ASN | MET | SER | ALA | ||||
| 9 | LEU | GLU | LEU | GLU | ASP | SER | ALA | MET | TYR | PHE | ||||
| 10 | CYS | ALA | SER | SER | LEU | ARG | TRP | GLY | ASP | GLU | ||||
| 11 | GLN | TYR | PHE | GLY | PRO | GLY | THR | ARG | LEU | THR | ||||
| 12 | VAL | LEU | GLU | ASP | LEU | ARG | ASN | VAL | THR | PRO | ||||
| 13 | PRO | LYS | VAL | SER | LEU | PHE | GLU | PRO | SER | LYS | ||||
| 14 | ALA | GLU | ILE | ALA | ASN | LYS | GLN | LYS | ALA | THR | ||||
| 15 | LEU | VAL | CYS | LEU | ALA | ARG | GLY | PHE | PHE | PRO | ||||
| 16 | ASP | HIS | VAL | GLU | LEU | SER | TRP | TRP | VAL | ASN | ||||
| 17 | GLY | LYS | GLU | VAL | HIS | SER | GLY | VAL | SER | THR | ||||
| 18 | ASP | PRO | GLN | ALA | TYR | LYS | GLU | SER | ASN | TYR | ||||
| 19 | SER | TYR | SER | LEU | SER | SER | ARG | LEU | ARG | VAL | ||||
| 20 | SER | ALA | THR | PHE | TRP | HIS | ASN | PRO | ARG | ASN | ||||
| 21 | HIS | PHE | ARG | CYS | GLN | VAL | GLN | PHE | HIS | GLY | ||||
| 22 | LEU | SER | GLU | GLU | ASP | LYS | TRP | PRO | GLU | GLY | ||||
| 23 | SER | PRO | LYS | PRO | VAL | THR | GLN | ASN | ILE | SER | ||||
| 24 | ALA | GLU | ALA | TRP | GLY | ARG | ALA | ASP | SER |
Samples:
sample_1: N15 beta TCR, [U-13C; U-15N; U-2H], 0.4 mM; sodium chloride 150 mM; sodium phosphate 50 mM; D2O 10%; H2O 90%
sample_conditions_1: ionic strength: 250 mM; pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA v1.90, Keller and Wuthrich - chemical shift assignment
NMR spectrometers:
- Bruker Avance 750 MHz
- Bruker Avance 600 MHz
- Varian INOVA 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts