BMRB Entry 4083
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4083
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Title: Assignments, Secondary Structure, Global Fold, and Dynamics of Chemotaxis Y Protein Using Three- and Four-Dimensional Heteronuclear (13C, 15N) NMR Spectroscopy
Deposition date: 1997-12-23 Original release date: 2001-02-17
Authors: Moy, Frankln; Lowry, David; Matsumura, Philip; Dahlquist, Frederick; Krywko, James; Domaille, Peter
Citation: Moy, Frankln; Lowry, David; Matsumura, Philip; Dahlquist, Frederick; Krywko, James; Domaille, Peter. "Assignments, Secondary Structure, Global Fold, and Dynamics of Chemotaxis Y Protein Using Three- and Four-Dimensional Heteronuclear (13C, 15N) NMR Spectroscopy" Biochemistry 33, 10731-10742 (1994).
Assembly members:
E. coli CheY, polymer, 128 residues, 24.305 Da.
MG, non-polymer, 24.305 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
E. coli CheY: ADKELKFLVVDDFSTMRRIV
RNLLKELGFNNVEEAEDGVD
ALNKLQAGGYGFVISDWNMP
NMDGLELLKTIRADGAMSAL
PVLMVTAEAKKENIIAAAQA
GASGYVVKPFTAATLEEKLN
KIFEKLGM
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 423 |
| 15N chemical shifts | 127 |
| 1H chemical shifts | 886 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CheY | 1 |
Entities:
Entity 1, CheY 128 residues - 24.305 Da.
| 1 | ALA | ASP | LYS | GLU | LEU | LYS | PHE | LEU | VAL | VAL | ||||
| 2 | ASP | ASP | PHE | SER | THR | MET | ARG | ARG | ILE | VAL | ||||
| 3 | ARG | ASN | LEU | LEU | LYS | GLU | LEU | GLY | PHE | ASN | ||||
| 4 | ASN | VAL | GLU | GLU | ALA | GLU | ASP | GLY | VAL | ASP | ||||
| 5 | ALA | LEU | ASN | LYS | LEU | GLN | ALA | GLY | GLY | TYR | ||||
| 6 | GLY | PHE | VAL | ILE | SER | ASP | TRP | ASN | MET | PRO | ||||
| 7 | ASN | MET | ASP | GLY | LEU | GLU | LEU | LEU | LYS | THR | ||||
| 8 | ILE | ARG | ALA | ASP | GLY | ALA | MET | SER | ALA | LEU | ||||
| 9 | PRO | VAL | LEU | MET | VAL | THR | ALA | GLU | ALA | LYS | ||||
| 10 | LYS | GLU | ASN | ILE | ILE | ALA | ALA | ALA | GLN | ALA | ||||
| 11 | GLY | ALA | SER | GLY | TYR | VAL | VAL | LYS | PRO | PHE | ||||
| 12 | THR | ALA | ALA | THR | LEU | GLU | GLU | LYS | LEU | ASN | ||||
| 13 | LYS | ILE | PHE | GLU | LYS | LEU | GLY | MET |
Samples:
sample_one: E. coli CheY, [U-15N], 1.0 – 4.0 mM; sodium phosphate 100 mM; magnesium chloride4 – 5 mM; sodium azide 0.02%; D2O 10%; H2O 90%
sample_two: E. coli CheY, [U-13C; U-15N], 1.0 – 4.0 mM; sodium phosphate 100 mM; magnesium chloride4 – 5 mM; sodium azide 0.02%; D2O 10%; H2O 90%
sample_three: E. coli CheY, [U-13C; U-15N], 1.0 – 4.0 mM; sodium phosphate 100 mM; magnesium chloride4 – 5 mM; sodium azide 0.02%; D2O 99.8%
sample_conditions: pH: 6.75; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | not available | not available | sample_conditions |
Software:
No software information available
NMR spectrometers:
- Bruker AMX 600 MHz
Related Database Links:
| BMRB | 2950 2951 3440 4472 |
| PDB | |
| DBJ | BAA15698 BAB36015 BAG77641 BAI25973 BAI30936 |
| EMBL | CAD05667 CAP76371 CAQ32359 CAQ98822 CAR03242 |
| GB | AAA23570 AAA23577 AAA27037 AAC74952 AAG56872 |
| PIR | AH0745 |
| REF | NP_310619 NP_416396 NP_456482 NP_460873 NP_707777 |
| SP | P0A2D5 P0A2D6 P0AE67 P0AE68 P0AE69 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts