BMRB Entry 4086
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4086
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Title: Tumor Suppressor p16ink4a: Structural Characterization of Wild-Type and Mutant Proteins by NMR and Circular Dichroism PubMed: 8755727
Deposition date: 1997-12-23 Original release date: 2002-07-17
Authors: Tevelev, Anton; Byeon, In-Ja; Selby, Thomas; Ericson, Karen; Kim, Hee-Jung; Kraynov, Vadim; Tsai, Ming-Daw
Citation: Tevelev, Anton; Byeon, In-Ja; Selby, Thomas; Ericson, Karen; Kim, Hee-Jung; Kraynov, Vadim; Tsai, Ming-Daw. "Tumor Suppressor p16ink4a: Structural Characterization of Wild-Type and Mutant Proteins by NMR and Circular Dichroism" Biochemistry 35, 9475-9487 (1996).
Assembly members:
p16(INK4)/delta_1-8, polymer, 148 residues, 16569 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
p16(INK4)/delta_1-8: MEPSADWLATAAARGRVEEV
RALLEAGALPNAPNSYGRRP
IQVMMMGSARVAELLLLHGA
EPNCADPATLTRPVHDAARE
GFLDTLVVLHRAGARLDVRD
AWGRLPVDLAEELGHRDVAR
YLRAAAGGTRGSNHARIDAA
EGPSDIPD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 330 |
| 15N chemical shifts | 123 |
| 1H chemical shifts | 684 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | p16(INK4)/delta_1-8 | 1 |
Entities:
Entity 1, p16(INK4)/delta_1-8 148 residues - 16569 Da.
| 1 | MET | GLU | PRO | SER | ALA | ASP | TRP | LEU | ALA | THR | ||||
| 2 | ALA | ALA | ALA | ARG | GLY | ARG | VAL | GLU | GLU | VAL | ||||
| 3 | ARG | ALA | LEU | LEU | GLU | ALA | GLY | ALA | LEU | PRO | ||||
| 4 | ASN | ALA | PRO | ASN | SER | TYR | GLY | ARG | ARG | PRO | ||||
| 5 | ILE | GLN | VAL | MET | MET | MET | GLY | SER | ALA | ARG | ||||
| 6 | VAL | ALA | GLU | LEU | LEU | LEU | LEU | HIS | GLY | ALA | ||||
| 7 | GLU | PRO | ASN | CYS | ALA | ASP | PRO | ALA | THR | LEU | ||||
| 8 | THR | ARG | PRO | VAL | HIS | ASP | ALA | ALA | ARG | GLU | ||||
| 9 | GLY | PHE | LEU | ASP | THR | LEU | VAL | VAL | LEU | HIS | ||||
| 10 | ARG | ALA | GLY | ALA | ARG | LEU | ASP | VAL | ARG | ASP | ||||
| 11 | ALA | TRP | GLY | ARG | LEU | PRO | VAL | ASP | LEU | ALA | ||||
| 12 | GLU | GLU | LEU | GLY | HIS | ARG | ASP | VAL | ALA | ARG | ||||
| 13 | TYR | LEU | ARG | ALA | ALA | ALA | GLY | GLY | THR | ARG | ||||
| 14 | GLY | SER | ASN | HIS | ALA | ARG | ILE | ASP | ALA | ALA | ||||
| 15 | GLU | GLY | PRO | SER | ASP | ILE | PRO | ASP |
Samples:
sample_one: p16(INK4)/delta_1-8 0.2 mM; HEPES 4 mM; DTT 1 mM; EDTA 5 uM; D2O 10%; H2O 90%
sample_two: p16(INK4)/delta_1-8 0.2 mM; HEPES 4 mM; DTT 1 mM; EDTA 5 uM; D2O 100%
sample_conditions: pH: 7.0; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- Bruker DMX 750 MHz
Related Database Links:
| BMRB | 4526 |
| PDB | |
| DBJ | BAA33541 BAB91133 |
| EMBL | CCQ43815 |
| GB | AAA92554 AAB32713 AAB60645 AAD14048 AAD14050 |
| PRF | 2002364A |
| REF | NP_000068 NP_001139762 NP_001182061 XP_003830733 XP_005251400 |
| SP | P42771 |
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