BMRB Entry 4091
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4091
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Title: 1H, 15N, 13C, and 13CO Assignments and Secondary Structure Determination of Basic Fibroblast Growth Factor using 3D Heteronuclear NMR Spectroscopy
Deposition date: 1998-01-06 Original release date: 1998-03-14
Authors: Moy, Franklin; Seddon, Andrew; Campbell, Ernest; Bohlen, Peter; Powers, Robert
Citation: Moy, Franklin; Seddon, Andrew; Campbell, Ernest; Bohlen, Peter; Powers, Robert. "1H, 15N, 13C, and 13CO Assignments and Secondary Structure Determination of Basic Fibroblast Growth Factor using 3D Heteronuclear NMR Spectroscopy" J. Biomol. NMR 6, 245-254 (1995).
Assembly members:
basic fibroblast Growth Factor, polymer, 154 residues, 17200 Da.
Natural source: Common Name: Human Taxonomy ID: not available Superkingdom: eucaryote Kingdom: Animalia Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
basic fibroblast Growth Factor: AEGEITTLPALPEDGGSGAF
PPGHFKDPKRLYCKNGGFFL
RIHPDGRVDGVREKSDPHIK
LQLQAEERGVVSIKGVSANR
YLAMKEDGRLLASKSVTDEC
FFFERLESNNYNTYRSRKYT
SWYVALKRTGQYKLGSKTGP
GQKAILFLPMSAKS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 686 |
| 15N chemical shifts | 151 |
| 1H chemical shifts | 1060 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FGF-2 | 1 |
Entities:
Entity 1, FGF-2 154 residues - 17200 Da.
| 1 | ALA | GLU | GLY | GLU | ILE | THR | THR | LEU | PRO | ALA | ||||
| 2 | LEU | PRO | GLU | ASP | GLY | GLY | SER | GLY | ALA | PHE | ||||
| 3 | PRO | PRO | GLY | HIS | PHE | LYS | ASP | PRO | LYS | ARG | ||||
| 4 | LEU | TYR | CYS | LYS | ASN | GLY | GLY | PHE | PHE | LEU | ||||
| 5 | ARG | ILE | HIS | PRO | ASP | GLY | ARG | VAL | ASP | GLY | ||||
| 6 | VAL | ARG | GLU | LYS | SER | ASP | PRO | HIS | ILE | LYS | ||||
| 7 | LEU | GLN | LEU | GLN | ALA | GLU | GLU | ARG | GLY | VAL | ||||
| 8 | VAL | SER | ILE | LYS | GLY | VAL | SER | ALA | ASN | ARG | ||||
| 9 | TYR | LEU | ALA | MET | LYS | GLU | ASP | GLY | ARG | LEU | ||||
| 10 | LEU | ALA | SER | LYS | SER | VAL | THR | ASP | GLU | CYS | ||||
| 11 | PHE | PHE | PHE | GLU | ARG | LEU | GLU | SER | ASN | ASN | ||||
| 12 | TYR | ASN | THR | TYR | ARG | SER | ARG | LYS | TYR | THR | ||||
| 13 | SER | TRP | TYR | VAL | ALA | LEU | LYS | ARG | THR | GLY | ||||
| 14 | GLN | TYR | LYS | LEU | GLY | SER | LYS | THR | GLY | PRO | ||||
| 15 | GLY | GLN | LYS | ALA | ILE | LEU | PHE | LEU | PRO | MET | ||||
| 16 | SER | ALA | LYS | SER |
Samples:
sample_one: basic fibroblast Growth Factor, [U-15N], 1 mM; DTT 10 mM; H2O 90%; D2O 10%; NaN3 2 mM; potassium phosphate 50 mM
sample_two: basic fibroblast Growth Factor, [U-15N; U-13C], 1 mM; DTT 10 mM; H2O 90%; D2O 10%; NaN3 2 mM; potassium phosphate 50 mM
sample_conditions_one: pH: 5.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- Bruker AM600 600 MHz
Related Database Links:
| BMRB | 18995 |
| PDB | |
| DBJ | BAG70135 BAG70264 |
| EMBL | CCO13911 CCO13913 |
| GB | AAA31248 AAA52448 AAA52531 AAA52532 AAA52533 |
| REF | NP_001103711 NP_001997 XP_001099284 XP_002815172 XP_003271404 |
| SP | P09038 P48799 Q5IS69 |
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