BMRB Entry 4094
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4094
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Title: 1H, 15N, and 13CO Assignments of Human Interleukin-4 Using Three Dimensional Double- and Triple-Resonance Heteronuclear Magnetic Resonance Spectroscopy
Deposition date: 1998-01-07 Original release date: 1998-03-02
Authors: Powers, Robert; Garrett, Daniel; March, Carl; Frieden, Eric; Gronenborn, Angela; Clore, G.
Citation: Powers, Robert; Garrett, Daniel; March, Carl; Frieden, Eric; Gronenborn, Angela; Clore, G.. "1H, 15N, and 13CO Assignments of Human Interleukin-4 Using Three Dimensional Double- and Triple-Resonance Heteronuclear Magnetic Resonance Spectroscopy" Biochemistry 31, 4334-4346 (1992).
Assembly members:
human Interleukin 4, polymer, 133 residues, 15400 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Saccharomyces cerevisiae
Entity Sequences (FASTA):
human Interleukin 4: EAEAHKCDITLQEIIKTLNS
LTEQKTLCTELTVTDIFAAS
KDTTEKETFCRAATVLRQFY
SHHEKDTRCLGATAQQFHRH
KQLIRFLKRLDRNLWGLAGL
NSCPVKEADQSTLENFLERL
KTIMREKYSKCSS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 605 |
| 15N chemical shifts | 144 |
| 1H chemical shifts | 963 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | IL-4 | 1 |
Entities:
Entity 1, IL-4 133 residues - 15400 Da.
| 1 | GLU | ALA | GLU | ALA | HIS | LYS | CYS | ASP | ILE | THR | ||||
| 2 | LEU | GLN | GLU | ILE | ILE | LYS | THR | LEU | ASN | SER | ||||
| 3 | LEU | THR | GLU | GLN | LYS | THR | LEU | CYS | THR | GLU | ||||
| 4 | LEU | THR | VAL | THR | ASP | ILE | PHE | ALA | ALA | SER | ||||
| 5 | LYS | ASP | THR | THR | GLU | LYS | GLU | THR | PHE | CYS | ||||
| 6 | ARG | ALA | ALA | THR | VAL | LEU | ARG | GLN | PHE | TYR | ||||
| 7 | SER | HIS | HIS | GLU | LYS | ASP | THR | ARG | CYS | LEU | ||||
| 8 | GLY | ALA | THR | ALA | GLN | GLN | PHE | HIS | ARG | HIS | ||||
| 9 | LYS | GLN | LEU | ILE | ARG | PHE | LEU | LYS | ARG | LEU | ||||
| 10 | ASP | ARG | ASN | LEU | TRP | GLY | LEU | ALA | GLY | LEU | ||||
| 11 | ASN | SER | CYS | PRO | VAL | LYS | GLU | ALA | ASP | GLN | ||||
| 12 | SER | THR | LEU | GLU | ASN | PHE | LEU | GLU | ARG | LEU | ||||
| 13 | LYS | THR | ILE | MET | ARG | GLU | LYS | TYR | SER | LYS | ||||
| 14 | CYS | SER | SER |
Samples:
sample_one: human Interleukin 4, [U-95% 15N], 2 mM; H2O 90%; D2O 10%
sample_two: human Interleukin 4, [U-95% 15N; U-95% 13C], 2 mM; D2O 99.996%
sample_conditions: pH: 5.7; temperature: 309 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- Bruker AM600 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
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