BMRB Entry 4110
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4110
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Title: 1H, 13C, 15N Resonance Assignment of the 20 KDa Double Stranded RNA Binding Domain of PKR
Deposition date: 1998-02-19 Original release date: 1998-10-14
Authors: Nanduri, Sambasivarao; Carpick, Bruce; Yang, Yan; Williams, Bryan; Qin, Jun
Citation: Nanduri, Sambasivarao; Carpick, Bruce; Yang, Yan; Williams, Bryan; Qin, Jun. "1H, 13C, 15N Resonance Assignment of the 20 KDa Double Stranded RNA Binding Domain of PKR" J. Biomol. NMR 12, 349-351 (1998).
Assembly members:
double stranded RNA binding domain of PKR, polymer, 179 residues, 20000 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
double stranded RNA binding domain of PKR: GSHMEMAGDLSAGFFMEELN
TRYQKQGVVLKYQELPNSGP
PHDRRFTFQVIIDGREFPEG
EGRSKKEAKNAAAKLAVEIL
NKEKKAVSPLLLTTTNSSEG
LSMGNYIGLINRIAQKKRLT
VNYEQCASGVHGPEGFHYKC
KMGQKEYSIGTGSTKQEAKQ
LAAKLAYLQILSEETGSGC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 577 |
| 15N chemical shifts | 176 |
| 1H chemical shifts | 1183 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | dsRBD-PKR | 1 |
Entities:
Entity 1, dsRBD-PKR 179 residues - 20000 Da.
| 1 | GLY | SER | HIS | MET | GLU | MET | ALA | GLY | ASP | LEU | ||||
| 2 | SER | ALA | GLY | PHE | PHE | MET | GLU | GLU | LEU | ASN | ||||
| 3 | THR | ARG | TYR | GLN | LYS | GLN | GLY | VAL | VAL | LEU | ||||
| 4 | LYS | TYR | GLN | GLU | LEU | PRO | ASN | SER | GLY | PRO | ||||
| 5 | PRO | HIS | ASP | ARG | ARG | PHE | THR | PHE | GLN | VAL | ||||
| 6 | ILE | ILE | ASP | GLY | ARG | GLU | PHE | PRO | GLU | GLY | ||||
| 7 | GLU | GLY | ARG | SER | LYS | LYS | GLU | ALA | LYS | ASN | ||||
| 8 | ALA | ALA | ALA | LYS | LEU | ALA | VAL | GLU | ILE | LEU | ||||
| 9 | ASN | LYS | GLU | LYS | LYS | ALA | VAL | SER | PRO | LEU | ||||
| 10 | LEU | LEU | THR | THR | THR | ASN | SER | SER | GLU | GLY | ||||
| 11 | LEU | SER | MET | GLY | ASN | TYR | ILE | GLY | LEU | ILE | ||||
| 12 | ASN | ARG | ILE | ALA | GLN | LYS | LYS | ARG | LEU | THR | ||||
| 13 | VAL | ASN | TYR | GLU | GLN | CYS | ALA | SER | GLY | VAL | ||||
| 14 | HIS | GLY | PRO | GLU | GLY | PHE | HIS | TYR | LYS | CYS | ||||
| 15 | LYS | MET | GLY | GLN | LYS | GLU | TYR | SER | ILE | GLY | ||||
| 16 | THR | GLY | SER | THR | LYS | GLN | GLU | ALA | LYS | GLN | ||||
| 17 | LEU | ALA | ALA | LYS | LEU | ALA | TYR | LEU | GLN | ILE | ||||
| 18 | LEU | SER | GLU | GLU | THR | GLY | SER | GLY | CYS |
Samples:
sample_one: double stranded RNA binding domain of PKR, [U-15N], .9 mM; NaCl 100 mM; sodium phosphate 20 mM; DTT 1 mM; H2O 93%; D2O 7%
sample_two: double stranded RNA binding domain of PKR, [U-15N;U-13C], .9 mM; NaCl 100 mM; sodium phosphate 20 mM; DTT 1 mM; H2O 93%; D2O 7%
sample_conditions_one: pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | not available | not available | sample_conditions_one |
Software:
NMRPipe - used for all data processing
PIPP - analysis of processed spectra
NMR spectrometers:
- Varian Inova 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAF83344 BAG36554 |
| GB | AAA18253 AAA36409 AAC50768 AAF13156 AAH07769 |
| REF | NP_001129123 NP_001129124 NP_001138509 NP_001266110 NP_001266614 |
| SP | P19525 |
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